丝状病毒检测与鉴定的快速分子策略
Rapid molecular strategy for filovirus detection and characterization.
作者信息
Zhai Junhui, Palacios Gustavo, Towner Jonathan S, Jabado Omar, Kapoor Vishal, Venter Marietjie, Grolla Allen, Briese Thomas, Paweska Janusz, Swanepoel Robert, Feldmann Heinz, Nichol Stuart T, Lipkin W Ian
机构信息
Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
出版信息
J Clin Microbiol. 2007 Jan;45(1):224-6. doi: 10.1128/JCM.01893-06. Epub 2006 Nov 1.
Abstract
Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.
摘要
丝状病毒能够在灵长类动物中引发致命的出血热疫情。在此，我们介绍一种用于丝状病毒识别和鉴定的简单一致性逆转录聚合酶链反应方法，并展示了其对所有已知丝状病毒毒株的适用性。通过基于网络的扩增产物自动化序列分析来实现系统发育分类。
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