Saudan Christophe, Augsburger Marc, Kintz Pascal, Saugy Martial, Mangin Patrice
Laboratoire Suisse d'Analyse du Dopage, Institut Universitaire de Médecine Légale, Rue du Bugnon 21, 1005 Lausanne, Switzerland.
J Anal Toxicol. 2005 Nov-Dec;29(8):777-81. doi: 10.1093/jat/29.8.777.
Because GHB (gamma-hydroxybutyrate) is present in both blood and urine of the general population, toxicologists must be able to discriminate between endogenous levels and a concentration resulting from exposure. In this paper, we propose a procedure for the detection of exogenous GHB in blood by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Following liquid-liquid and solid-phase extractions, GHB is derivatized to GHB di-TMS before analysis by GC-C-IRMS. Significant differences in the carbon isotopic ratio (delta(13)C-values > 13.5 per thousand) were found between endogenous and synthetic GHB. Indeed, for postmortem blood samples with different GHB concentrations (range: 13.8-86.3 mg/L), we have obtained GHB delta(13)C-values ranging from -20.6 to -24.7 per thousand, whereas delta(13)C-values for the GHB from police seizure were in the range -38.2 to -50.2 per thousand. In contrast to the use of cut-off concentrations for positive postmortem blood GHB concentrations, this method should provide an unambiguous indication of the drug origin.
由于普通人群的血液和尿液中均存在γ-羟基丁酸（GHB），毒理学家必须能够区分内源性水平和因接触而产生的浓度。在本文中，我们提出了一种通过气相色谱-燃烧-同位素比值质谱法（GC-C-IRMS）检测血液中外源性GHB的方法。经过液-液萃取和固相萃取后，GHB在通过GC-C-IRMS分析之前被衍生化为GHB二-TMS。内源性GHB和合成GHB之间的碳同位素比值存在显著差异（δ¹³C值>13.5‰）。事实上，对于不同GHB浓度（范围：13.8 - 86.3 mg/L）的死后血液样本，我们获得的GHB δ¹³C值范围为-20.6至-24.7‰，而警方查获的GHB的δ¹³C值范围为-38.2至-50.2‰。与使用死后血液GHB阳性浓度的截断浓度不同，该方法应能明确指示药物来源。
