基于带有猪肝细胞的无纺布生物反应器的生物人工肝在猪体内的评估。

Evaluation of a bioartificial liver based on a nonwoven fabric bioreactor with porcine hepatocytes in pigs.

作者信息

Li Lan Juan, Du Wei Bo, Zhang Yi Min, Li Jun, Pan Xiao Ping, Chen Jia Jia, Cao Hong Cui, Chen Yu, Chen Yue Mei

机构信息

Key Laboratory of Infectious Diseases, Ministry of Public Health, Department of Infectious Diseases, The 1st Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

出版信息

J Hepatol. 2006 Feb;44(2):317-24. doi: 10.1016/j.jhep.2005.08.006. Epub 2005 Sep 16.

DOI:10.1016/j.jhep.2005.08.006

PMID:16356580

Abstract

BACKGROUND/AIMS: We developed a bioartificial liver (BAL) based on a direct hemoperfusion typed nonwoven fabric bioreactor containing porcine hepatocytes. In this study, the efficacy of our BAL was evaluated with a pig fulminant hepatic failure (FHF) model.

METHODS

FHF was induced with intravenous administration of D-galactosamine (1.3 g/kg) in each pig. Twelve hours post D-galactosamine injection, fifteen pigs were divided into: a BAL group (n = 5), in which pigs received the BAL treatment with 1.0 to 1.3 x 10(9) hepatocytes for 6 h, a sham BAL group (n = 5), in which pigs received the BAL treatment without hepatocytes, and a FHF group (n = 5), in which pigs only received intensive care. Parameters related to liver function and animal survival up to 168 h were determined.

RESULTS

In the BAL group, blood ammonia and plasma lactate levels were lower, and serum glucose levels and Fischer index were higher than those in the other two groups. Survival time of pigs in the BAL group was significantly prolonged as compared with the sham BAL and the FHF group.

CONCLUSIONS

The BAL based on a nonwoven fabric bioreactor containing porcine hepatocytes appears to be effective in the treatment of FHF in pigs.

摘要

背景/目的：我们开发了一种基于含有猪肝细胞的直接血液灌流型无纺布生物反应器的生物人工肝（BAL）。在本研究中，我们用猪暴发性肝衰竭（FHF）模型评估了我们的BAL的疗效。

方法

每头猪静脉注射D-半乳糖胺（1.3 g/kg）诱导FHF。在注射D-半乳糖胺12小时后，将15头猪分为：BAL组（n = 5），猪接受含有1.0至1.3×10⁹个肝细胞的BAL治疗6小时；假BAL组（n = 5），猪接受无肝细胞的BAL治疗；FHF组（n = 5），猪仅接受重症监护。测定与肝功能和动物存活至168小时相关的参数。