Naruse K, Sakai Y, Lei G, Sakamoto Y, Kobayashi T, Puliatti C, Aronica G, Morale W, Leone F, Qiang S, Ming S G, Ming S, Li Z, Chang S J, Suzuki M, Makuuchi M
Division of Artificial Organs and Transplantation, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Artif Organs. 2001 Apr;25(4):273-80.
We have developed a new bioartificial liver bioreactor filled with porcine hepatocytes immobilized on polyester nonwoven fabric (NWF). In this study, we investigated the efficacy of our hybrid bioartificial liver system incorporating the NWF bioreactors and an immunoglobulin adsorbent column for perfusion treatment in a canine liver failure model. Xenogeneic perfusion treatment for operative canine liver failure models were performed for 3 h, and survival time, intracranial pressure, and blood and cerebrospinal fluid data were documented. Treatment was carried out without obstruction by immunological rejection when immunoglobulin adsorbent columns were used with the NWF bioreactors in combination. Dogs treated with this system exhibited a restricted increase of intracranial pressure and significant compensatory effects on blood and cerebrospinal amino acid imbalances as shown by a significant improvement of Fischer's ratio. On the other hand, relatively low capacity for ammonia elimination was shown as compared with homologous direct hemoperfusion.
我们研发了一种新型生物人工肝生物反应器，其内部填充有固定在聚酯无纺布（NWF）上的猪肝细胞。在本研究中，我们调查了我们的混合生物人工肝系统（该系统包含NWF生物反应器和免疫球蛋白吸附柱）在犬肝衰竭模型中进行灌注治疗的效果。对手术造成的犬肝衰竭模型进行了3小时的异种灌注治疗，并记录了存活时间、颅内压以及血液和脑脊液数据。当免疫球蛋白吸附柱与NWF生物反应器联合使用时，治疗未因免疫排斥而受阻。用该系统治疗的犬颅内压升高受限，对血液和脑脊液氨基酸失衡具有显著的代偿作用，费舍尔比率有显著改善。另一方面，与同种直接血液灌注相比，氨清除能力相对较低。
