Albright A L, Johnson P R, Greene S, Stern J S
Department of Nutrition, University of California, Davis, CA 95616, USA.
Obes Res. 1994 Nov;2(6):535-9. doi: 10.1002/j.1550-8528.1994.tb00102.x.
The Wistar Diabetic Fatty rat (WDF fafa) is a congenic strain of the Wistar Kyoto rat. Studies using blood glucose reveal that only fatty male (not female) WDF rats spontaneously develop hyperglycemia when fed a stock diet. Blood glucose values have not provided consistent results for evaluation of glycemic status in fatty male WDF rats. Zucker fatty (fafa) rats, while sharing the fa gene and the development of hyperinsulinemia and hyperlipemia, do not spontaneously become hyperglycemic. In order to examine strain differences and the effects of age on long-term average glycemic status in WDF and Zucker rats, glycated hemoglobin (GHb) was analyzed. Glycated hemoglobin was measured in male lean and obese WDF and Zucker rats at 2, 3, 6, and 12 months of age. Nonfasted plasma glucose was measured in male lean and obese WDF rats at 2, 3, 6, and 12 months of age and in lean and obese Zucker rats at 3, 6, and 12 months of age. Plasma insulin was measured in lean and obese WDF and Zucker rats at 3, 6, and 12 months of age. Obese WDF rats had significantly elevated GHb compared to lean controls at 3, 6, and 12 months of age. Glycated hemoglobin was substantially above the normal range (3.8-6.5%) at 3 months of age (14.1%). Glycated hemoglobin significantly declined in the obese WDF rats between 6 and 12 months of age. Nonfasted plasma glucose was significantly elevated in the obese WDF rats at 3 months (14.1 +/- 2.1 mM/L) and 6 months of age (16.2 +/- 2.3 mM/L) compared to lean controls. At 12 months of age there was no difference in plasma glucose between obese and lean WDF rats. Obese and lean Zucker rats had similar levels of GHb and plasma glucose at all ages. In conclusion, GHb provides more integrated data for classifying disease status of WDF rats and evaluation of potential long-term complications associated with hyperglycemia.
Wistar糖尿病脂肪大鼠（WDF fafa）是Wistar京都大鼠的一个同源品系。使用血糖进行的研究表明，仅雄性（而非雌性）脂肪型WDF大鼠在喂食常规饲料时会自发出现高血糖。血糖值在评估雄性脂肪型WDF大鼠的血糖状态时并未提供一致的结果。Zucker脂肪大鼠（fafa）虽然共享fa基因且会出现高胰岛素血症和高脂血症，但不会自发出现高血糖。为了研究WDF和Zucker大鼠的品系差异以及年龄对长期平均血糖状态的影响，对糖化血红蛋白（GHb）进行了分析。在2、3、6和12月龄的雄性瘦型和肥胖型WDF和Zucker大鼠中测量了糖化血红蛋白。在2、3、6和12月龄的雄性瘦型和肥胖型WDF大鼠以及3、6和12月龄的瘦型和肥胖型Zucker大鼠中测量了非空腹血糖。在3、6和12月龄的瘦型和肥胖型WDF和Zucker大鼠中测量了血浆胰岛素。在3、6和12月龄时，肥胖型WDF大鼠的GHb水平与瘦型对照相比显著升高。在3月龄时，糖化血红蛋白大幅高于正常范围（3.8 - 6.5%），为14.1%。肥胖型WDF大鼠的糖化血红蛋白在6至12月龄之间显著下降。与瘦型对照相比，肥胖型WDF大鼠在3月龄（14.1 +/- 2.1 mM/L）和6月龄（16.2 +/- 2.3 mM/L）时非空腹血糖显著升高。在12月龄时，肥胖型和瘦型WDF大鼠的血糖没有差异。在所有年龄段，肥胖型和瘦型Zucker大鼠的GHb和血糖水平相似。总之，GHb为分类WDF大鼠的疾病状态以及评估与高血糖相关的潜在长期并发症提供了更综合的数据。
