Martin J E, Philpott J, Chatkoff M, Young R, Pauerstein C J
Am J Obstet Gynecol. 1975 Jan 15;121(2):180-6. doi: 10.1016/0002-9378(75)90636-5.
The macromolecular binding of estradiol was investigated in the rabbit uterus under various hormonal states in a search for evidence of progesterone antagonism of the estrogen-receptor complex interaction. Progesterone did not alter the sucrose gradient sedimentation characteristics of the estrogen-receptor complex in estrus or castrate animals. Progesterone treatment of castrated animals did not alter the absolute number of EBS (estrogen-binding sites) per cell, but did result in decreased relative concentration of EBS by increasing the soluble protein content of the cells over that seen in untreated castrates. Castration did not alter the absolute number of EBS per cell but did increase the relative concentration of estrogen binding in the cytosol.
在不同激素状态下，对兔子宫中雌二醇的大分子结合进行了研究，以寻找孕酮对雌激素 - 受体复合物相互作用产生拮抗作用的证据。在发情期或去势动物中，孕酮并未改变雌激素 - 受体复合物的蔗糖梯度沉降特性。对去势动物进行孕酮处理，并未改变每个细胞中雌激素结合位点（EBS）的绝对数量，但通过增加细胞中可溶性蛋白质含量，导致EBS的相对浓度降低，相较于未处理的去势动物。去势并未改变每个细胞中EBS的绝对数量，但确实增加了细胞质中雌激素结合的相对浓度。
