Voloshin S A, Kaprel'iants A S
Prikl Biokhim Mikrobiol. 2005 Nov-Dec;41(6):647-51.
Cell aggregation was studied using the method of dynamic light scattering in the course of growth of Micrococcus luteus cultures in a liquid medium. The method detects particles ranging in size from 0.5 to 1000 microm in samples containing no more than 10(5) cells/ml. When grown in liquid media, M. luteus forms aggregates; during the lag phase, 80% of the cells are found in aggregates of 10 to 1000 microm, only minor amounts being represented by single cells. With the onset of exponential growth, the aggregates were decomposed, and single cells became prevalent in the culture liquid. This observation confirms that the aggregation of the cells during the lag phase is prerequisite to the initiation of bacterial growth. The method may be used in biotechnology for monitoring the state of bacterial cultures.
在黄色微球菌在液体培养基中的生长过程中，使用动态光散射法研究了细胞聚集情况。该方法可检测样品中大小在0.5至1000微米范围内、细胞浓度不超过10⁵个细胞/毫升的颗粒。当在液体培养基中生长时，黄色微球菌会形成聚集体；在迟缓期，80%的细胞存在于10至1000微米的聚集体中，单细胞仅占少量。随着指数生长期的开始，聚集体分解，单细胞在培养液中变得普遍。这一观察结果证实，迟缓期细胞的聚集是细菌生长起始的先决条件。该方法可用于生物技术中监测细菌培养物的状态。
