Monaselidze Dzh, Kiladze M, Barbakadze Sh, Kvavadze R, Chkhaidze M, Gelazonia L, Svanidze I
Biofizika. 2005 Nov-Dec;50(6):1131-6.
The denaturation heat parameters of hippocampus and olfactory bulb nodulus tissues were determined. The total denaturation heat calculated from the areas of endotherms I-IX onto which the dependence deltaH = f(T) is factorized equals to 13.03 +/- 1.3 J/g for bulb nodules and 9.91 J/g for the hippocampus. It was shown that chromatin in the composition of tissues had two stages of denaturation with the following parameters: for bulb nodules: Td(VIII) = 99.4 degrees C, Qd(VIII) = 62.3 +/- 0.8 J/g DNA, Td = (IX) = 106 degrees C, Qd = 28.8 ; 0.4 J/g DNA; and for hippocampus: Td(VIII) = 95 degrees C; Qd(VIII) = 62.0 +/- 9 J/g. Td(IX) - 107 degrees C; Qd(IX) = 29.0 +/- 4.5 J/g DNA. It was established that, along with denaturation of cytoplasmatic structures, nonhistone, nuclear proteins and damage to the nuclear matrix, toluene caused the redistribution of heat between endotherms IX, VIII, VIII(I) connected with the infolding of chromatin loops and 30-nm fibers. It is supposed that toluene causes the damage to the genetic material due to not only its oxidative influence on chromatin DNA but also the disturbance of nuclear matrix structural organization and partial denaturation of nuclear proteins of non-histone origin.
测定了海马体和嗅球小结组织的变性热参数。根据将依赖性ΔH = f(T)分解后的吸热峰I - IX的面积计算出的总变性热，嗅球小结为13.03±1.3 J/g，海马体为9.91 J/g。结果表明，组织成分中的染色质有两个变性阶段，其参数如下：嗅球小结：Td(VIII)=99.4℃，Qd(VIII)=62.3±0.8 J/g DNA，Td(IX)=106℃，Qd = 28.8；0.4 J/g DNA；海马体：Td(VIII)=95℃；Qd(VIII)=62.0±9 J/g。Td(IX)=107℃；Qd(IX)=29.0±4.5 J/g DNA。已确定，除了细胞质结构、非组蛋白、核蛋白变性以及核基质受损外，甲苯还导致了与染色质环和30纳米纤维折叠相关的吸热峰IX、VIII、VIII(I)之间的热重新分布。据推测，甲苯不仅通过对染色质DNA的氧化作用，还通过扰乱核基质的结构组织以及非组蛋白来源的核蛋白的部分变性，对遗传物质造成损害。
