Lialikau S A, Haurylik L L, Sobieska M, Klachko N M, Samborski W
State Medical University, Grodno, Belarus.
Rocz Akad Med Bialymst. 2005;50:279-83.
The paper presents links between iodine provision and selected acute phase proteins' (APP) serum concentrations as well as their glycosylations profiles (investigated with the use of affinity immunoelectrophoresis with Concanavalin A as ligand) in children.
116 children (58 girls and 58 boys) were enrolled. Iodine level was measured in the morning (7:30-8:30) urine portion, using Cr-As method. According to iodine level children were divided into two groups. The first one consisted of 56 children with decreased iodine level (lower than 100 micrograms/L), second--60 children with iodine level higher than 100 micrograms/L. In serum the concentration of ferritin, beta2-microglobulin (beta2-MG), thyroxin (T4), triiodothyronin (T3), thyrotrophic hormone (TSH) were measured by radioimmunoassay (BELORIS, Belarus). Concentrations of APP: C-reactive protein (CRP), alphal-acid glycoprotein (AGP), alphal-antichymotrypsin (ACT), alphal-antitrypsin (AT), haptoglobin (Hp), alpha2-macroglobulin (A2-M), ceruloplasmin (Cp) and transferrin (Tf) were measured in sera samples by rocket immunoelectrophoresis acc. to Laurell with antibodies and standard from DakoCytomation, Denmark. Microheterogeneity of AGP, ACT and Tf was estimated using affinity immunoelectrophoresis with ConA as a ligand, acc. to Bøg-Hansen.
It was established, that CRP level was lower than upper limit of normal range. Levels of other investigated proteins were reliably dependent on the level of iodine. Especially for AGP lower level was observed for children of the group with low iodine level. In children with low iodine level along with the decrease of serum AGP concentration altered glycosylations profile was observed, namely decrease in the content of variant non-reactive to ConA (W0) and increase in content of weakly reactive (W1) and reactive (W2) variants content, which resulted in increase of the reactivity coefficient (AGP-RC). Similar tendency in alterations of distinctly glycosylated variants in relation to iodine level could be shown for ACT. Serum concentration of any investigated protein was not dependent on the concentration of the hormones of pituitary-thyroid system.
It seems that the influence of the iodine level is direct, not via thyroid hormones. It could be suggested that in euthyroid children with low iodine excretion with urine a hidden iodine deficiency is already registered by the regulatory mechanisms and a kind of acute phase reaction is started, may be in order to increase iodine uptake and storage.
本文介绍了儿童碘供应与选定的急性期蛋白（APP）血清浓度及其糖基化谱（使用伴刀豆球蛋白A作为配体的亲和免疫电泳进行研究）之间的联系。
招募了116名儿童（58名女孩和58名男孩）。使用铬-砷法在早晨（7:30 - 8:30）的尿液样本中测量碘水平。根据碘水平将儿童分为两组。第一组由56名碘水平降低（低于100微克/升）的儿童组成，第二组由60名碘水平高于100微克/升的儿童组成。通过放射免疫分析法（白俄罗斯的BELORIS）测量血清中铁蛋白、β2-微球蛋白（β2-MG）、甲状腺素（T4）、三碘甲状腺原氨酸（T3）、促甲状腺激素（TSH）的浓度。通过火箭免疫电泳，根据劳雷尔法，使用丹麦DakoCytomation公司的抗体和标准品，测量血清样本中APP的浓度：C反应蛋白（CRP）、α1-酸性糖蛋白（AGP）、α1-抗糜蛋白酶（ACT）α1-抗胰蛋白酶（AT）、触珠蛋白（Hp）、α2-巨球蛋白（A2-M）、铜蓝蛋白（Cp）和转铁蛋白（Tf）。根据Bøg-Hansen法，使用伴刀豆球蛋白A作为配体的亲和免疫电泳估计AGP、ACT和Tf的微异质性。
已确定CRP水平低于正常范围上限。其他研究蛋白的水平可靠地依赖于碘水平。特别是对于AGP，碘水平低的儿童组中观察到较低水平。在碘水平低的儿童中，随着血清AGP浓度的降低，观察到糖基化谱发生改变，即对伴刀豆球蛋白A无反应的变体（W0）含量减少，弱反应（W1）和反应性（W2）变体含量增加，这导致反应系数（AGP-RC）增加。对于ACT，也可以显示出与碘水平相关的明显糖基化变体的类似变化趋势。任何研究蛋白的血清浓度均不依赖于垂体-甲状腺系统激素的浓度。
似乎碘水平的影响是直接的，而非通过甲状腺激素。可以推测，在尿碘排泄低的甲状腺功能正常的儿童中，调节机制已经察觉到隐匿性碘缺乏，并开始了一种急性期反应，可能是为了增加碘的摄取和储存。
