Pucher Beata, Sobieska Magdalena, Steiner Iwona, Grzegorowski Michał, Wiktorowicz Krzysztof
Klinika Otolaryngologii Dzieciecej Katedry Otolaryngologii AM w Poznaniu.
Otolaryngol Pol. 2006;60(5):743-6.
Acute subglottic laryngitis (pseudocroup) usually occurs in infants and small children between 1 and 3 years of age. Due to the viral etiology there are autumn-winter and winter-spring peaks of frequency of this disease observed. Acute-phase proteins are a group of proteins whose concentration in the serum changes following variety of injuries such as bacterial, viral or parasitic infection. These changes are one of the features of an early, non-specific and highly complex reaction of the organism called acute-phase response. The purpose of this reaction is to restore homeostasis. Almost all acute-phase proteins are glycoproteins and changes in their microheterogeneity (glycosylation profile: structure of side suger chains) were also reported during different inflammatory conditions.
51 children admitted to the Pediatric ENT Dept in Poznan because of an incident of pseudocroup were divided into 3 groups according to their age: I (0-3 years of age), II (between 3 and 5 years old) and III (over 5 years old). In all children levels of selected acute-phase proteins such as a1-antitrypsin [AT], al-antichymotrypsin [ACT], al-acid glycoprotein [AGP], ceruloplasmin [Cp], transferrin [Tf] and haptoglobin [Hp] were measured in three time points: at the beginning of the disease, after treatment and 3 weeks later during control examination. In all sera samples also glycosylation profile of AGP, ACT and Tf was performed.
Analysis of AGP glycosylation profile revealed the highest levels of microheterogeneity variants: W2 and W3 in the group of children over 5 years old. Also AGP-RC was significantly increased in this group. The Tf variant T4 reached the highest level in children between 3 and 5 years of age. In all age groups increased level of Hp was observed during treatment and decreased 3 weeks later during control examination.
Acute-phase reaction is the most intensive in the youngest children. Analysis of glycosylation profile revealed acute inflammatory process during episode of pseudocroup in the oldest children (over 5 years old) and chronic inflammation in group of the youngest children.
急性声门下喉炎（假膜性喉炎）通常发生于1至3岁的婴幼儿。由于其病毒病因，该病在秋冬和冬春季节发病率出现高峰。急性期蛋白是一组蛋白质，其血清浓度在各种损伤（如细菌、病毒或寄生虫感染）后会发生变化。这些变化是机体早期、非特异性且高度复杂反应（称为急性期反应）的特征之一。该反应的目的是恢复体内平衡。几乎所有急性期蛋白都是糖蛋白，在不同炎症状态下也报道了它们的微观异质性（糖基化谱：侧糖链结构）的变化。
51名因假膜性喉炎事件入住波兹南儿科耳鼻喉科的儿童，根据年龄分为3组：I组（0至3岁）、II组（3至5岁）和III组（5岁以上）。在所有儿童中，在三个时间点测量选定的急性期蛋白水平，如α1 - 抗胰蛋白酶[AT]、α1 - 抗糜蛋白酶[ACT]、α1 - 酸性糖蛋白[AGP]、铜蓝蛋白[Cp]、转铁蛋白[Tf]和触珠蛋白[Hp]：疾病开始时、治疗后以及3周后的对照检查时。在所有血清样本中还检测了AGP、ACT和Tf的糖基化谱。
AGP糖基化谱分析显示，5岁以上儿童组中微观异质性变体W2和W3的水平最高。该组中AGP - RC也显著增加。Tf变体T4在3至5岁儿童中达到最高水平。在所有年龄组中，治疗期间Hp水平升高，3周后的对照检查时降低。
急性期反应在最年幼的儿童中最为强烈。糖基化谱分析显示，年龄最大的儿童（5岁以上）假膜性喉炎发作期间存在急性炎症过程，而最年幼的儿童组存在慢性炎症。
