Handal Maria I, Ugaz Victor M
Texas A&M University, Department of Chemical Engineering, College Station, TX 77843-3122, USA.
Expert Rev Mol Diagn. 2006 Jan;6(1):29-38. doi: 10.1586/14737159.6.1.29.
Identification of genetic sequence variations occurring on a population-wide scale is key to unraveling the complex interactions that are the underlying cause of many medical disorders and diseases. A critical need exists, however, for advanced technology to enable DNA mutation analysis to be performed with significantly higher throughput and at significantly lower cost than is currently attainable. Microfluidic systems offer an attractive platform to address these needs by combining the ability to perform rapid analysis with a simplified device format that can be inexpensively mass-produced. This paper will review recent progress toward developing these next-generation systems and discuss challenges associated with adapting these technologies for routine laboratory use.
识别全人群范围内发生的基因序列变异是解开许多医学病症和疾病潜在病因的复杂相互作用的关键。然而，目前迫切需要先进技术，以便能够以比现有水平显著更高的通量和更低的成本进行DNA突变分析。微流控系统提供了一个有吸引力的平台来满足这些需求，它将快速分析能力与可低成本大规模生产的简化设备形式相结合。本文将回顾开发这些下一代系统的最新进展，并讨论将这些技术应用于常规实验室使用所面临的挑战。
