Thomas Gloria A, Farquar Hannah D, Sutton Shelby, Hammer Robert P, Soper Steven A
National Institute of Standards and Technology, Chemical Science and Technology Laboratory, 100 Bureau Drive, MS 8362, Gaithersburg, MD 20899, USA.
Expert Rev Mol Diagn. 2002 Sep;2(5):429-47. doi: 10.1586/14737159.2.5.429.
Biomedical microelectromechanical systems (BioMEMS) are rapidly emerging in many areas of genetic analysis. These devices demonstrate potential for rapid analysis using modular components capable of sample purification, amplification, mutation discrimination and detection on small, portable point-of-care instruments. Here, various approaches to genetic mutation detection and the modern analysis platform, capillary electrophoresis, will be briefly reviewed. Microfluidic devices will be discussed in relation to fabrication techniques, mutation detection using simple electrophoretic separations, multiplexed designs and modular functionalities, as well as challenges and issues surrounding this technology.
生物医学微机电系统（BioMEMS）正在遗传分析的许多领域迅速兴起。这些设备展示了使用模块化组件进行快速分析的潜力，这些组件能够在小型便携式即时检测仪器上进行样品纯化、扩增、突变鉴别和检测。在这里，将简要回顾基因突变检测的各种方法以及现代分析平台——毛细管电泳。将讨论微流控设备的制造技术、使用简单电泳分离的突变检测、多重设计和模块化功能，以及围绕该技术的挑战和问题。
