Henriksen Jens, Roepstorff Peter, Ringborg Lene Hoffmeyer
Department of Biochemistry and Molecular Biology, University of Southern Denmark, 55 Campusvej, DK-5230 Odense M, Denmark.
Carbohydr Res. 2006 Feb 27;341(3):382-7. doi: 10.1016/j.carres.2005.11.030. Epub 2005 Dec 19.
Heparin and heparin-derived components are widely applied anticoagulant drugs used for amongst other applications medical treatment of deep vein thrombosis and pulmonary embolism. Depolymerisation of native heparin results in complex mixtures of sulfated linear oligosaccharides that are usually not well characterised. In order to further characterise such mixtures, two on-line ion-pairing reverse-phased chromatography electrospray ionisation (ESI) mass spectrometry methods have been developed. One of the systems allows the determination of more than 200 components in a medium molecular weight heparin preparation, whereas the other system can be used to separate isomeric heparin oligosaccharides after previous separation according to size. This latter system allows semi-preparative isolation of isomeric heparin oligosaccharides. The experimental setup includes on-line cation exchange in order to prevent the ion-pairing reagent from entering the mass spectrometer.
肝素及肝素衍生成分是广泛应用的抗凝药物，用于治疗深静脉血栓形成和肺栓塞等多种疾病。天然肝素的解聚会产生硫酸化线性寡糖的复杂混合物，这些混合物通常没有得到很好的表征。为了进一步表征此类混合物，已开发出两种在线离子对反相色谱电喷雾电离（ESI）质谱方法。其中一种系统可测定中分子量肝素制剂中的200多种成分，而另一种系统可用于在按大小预先分离后分离异构肝素寡糖。后一种系统允许对异构肝素寡糖进行半制备分离。实验装置包括在线阳离子交换，以防止离子对试剂进入质谱仪。
