Duran Metin, Haznedaroğlu Berat Z, Zitomer Daniel H
Department of Civil and Environmental Engineering, Villanova University, Villanova, PA 19010, USA.
Water Res. 2006 Jan;40(1):67-74. doi: 10.1016/j.watres.2005.10.019. Epub 2005 Dec 19.
The objective of this study was to investigate the host-specific differences in fatty acid methyl ester (FAME) profiles of fecal coliforms (FC). A known-source library was constructed with 314 FC isolates cultured from 6 possible sources of fecal pollution; 99 isolates from sewage; 29 from bovine; 29 from poultry; 50 from swine; 46 from waterfowl; and 61 from deer. It was found that the hydroxy FAMEs 12:0 2 OH, 12:03 OH, and 14:02 OH were exclusively associated with isolates of human origin. On the other hand, 3 saturated FAMEs, 10:0, 15:0, and 18:0 were found only in isolates from non-human sources, 15:0 being associated with livestock samples only. In addition to the presence of these signature FAMEs, the mean relative masses of 16:1 omega7c and 16:1 ISO/14:03 OH were significantly different between the isolates of human and non-human origins. A linear discriminant function differentiated FC isolates of human origin from those of livestock and wildlife origin at 99% accuracy. These results strongly suggest that the FAME profiles of FC show statistically significant host specificity and may have the potential to be used as a phenotypic microbial source tracking tool.
本研究的目的是调查粪大肠菌群（FC）脂肪酸甲酯（FAME）谱的宿主特异性差异。构建了一个已知来源文库，其中包含从6种可能的粪便污染源培养的314株FC分离株；99株来自污水；29株来自牛；29株来自家禽；50株来自猪；46株来自水禽；61株来自鹿。研究发现，羟基FAMEs 12:0 2 OH、12:0 3 OH和14:0 2 OH仅与人类来源的分离株相关。另一方面，3种饱和FAMEs，10:0、15:0和18:0仅在非人类来源的分离株中发现，15:0仅与家畜样本相关。除了这些特征性FAMEs的存在外，人类和非人类来源分离株中16:1 omega7c和16:1 ISO/14:0 3 OH的平均相对质量也存在显著差异。线性判别函数以99%的准确率区分人类来源的FC分离株与家畜和野生动物来源的分离株。这些结果有力地表明，FC的FAME谱具有统计学上显著的宿主特异性，可能有潜力用作表型微生物源追踪工具。
