Yamasaki Hiroshi, Nakao Minoru, Sako Yasuhito, Nakaya Kazuhiro, Sato Marcello Otake, Ito Akira
Department of Parasitology, Asahikawa Medical College, Japan.
Parasitol Int. 2006;55 Suppl:S81-5. doi: 10.1016/j.parint.2005.11.013. Epub 2005 Dec 15.
Molecular diagnosis for taeniasis and cysticercosis in humans on the basis of mitochondrial DNA analysis was reviewed. Development and application of three different methods, including restriction fragment length polymorphism analysis, base excision sequence scanning thymine-base analysis and multiplex PCR, were described. Moreover, molecular diagnosis of cysticerci found in specimens submitted for histopathology and the molecular detection of taeniasis using copro-DNA were discussed.
综述了基于线粒体DNA分析的人体绦虫病和囊尾蚴病的分子诊断。描述了三种不同方法的开发和应用，包括限制性片段长度多态性分析、碱基切除序列扫描胸腺嘧啶碱基分析和多重PCR。此外，还讨论了在提交进行组织病理学检查的标本中发现的囊尾蚴的分子诊断以及使用粪便DNA对绦虫病进行的分子检测。
