Nassif Nader A, Armstrong William B, de Boer Johannes F, Wong Brian J F
Department of Physics and Astronomy, University of California, Irvine, Orange, CA 92868-3201, USA.
Otolaryngol Head Neck Surg. 2005 Dec;133(6):845-50. doi: 10.1016/j.otohns.2005.09.013.
To evaluate the ability of optical coherence tomography (OCT) (a noncontact, high-resolution imaging technique that generates detailed cross-sectional images of tissue structure) to image and document acute response to trauma in the larynx.
Porcine larynges were dissected to expose the vocal cords. Vocal cords were imaged with 1310 nanometer (nm) light source with a bandwidth of 75 nm at 1 frame/second (s). Vocal cord injuries were simulated by applying topical phenolic acid, injecting titanium dioxide into the submucosa, and carbon dioxide laser irradiation.
The epithelium, basement membrane, and lamina propria were clearly delineated, and the effect of each intervention could be monitored as lesions progressed. The OCT image corresponded closely with histology.
OCT is a powerful imaging tool with the potential for use in real time and has potential for multiple clinical applications in the larynx.
评估光学相干断层扫描（OCT）（一种非接触式高分辨率成像技术，可生成组织结构的详细横截面图像）对喉部创伤进行成像及记录急性反应的能力。
解剖猪喉以暴露声带。使用带宽为75纳米（nm）的1310纳米（nm）光源，以每秒1帧（s）的速度对声带进行成像。通过局部应用酚酸、向黏膜下层注射二氧化钛以及二氧化碳激光照射来模拟声带损伤。
上皮、基底膜和固有层清晰可辨，随着病变进展可监测到每种干预措施的效果。OCT图像与组织学结果密切相符。
OCT是一种强大的成像工具，具有实时使用的潜力，在喉部有多种临床应用潜力。
