Matsuo Eiko, Tani Hideki, Lim Chang kweng, Komoda Yasumasa, Okamoto Toru, Miyamoto Hironobu, Moriishi Kohji, Yagi Shintaro, Patel Arvind H, Miyamura Tatsuo, Matsuura Yoshiharu
Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Biochem Biophys Res Commun. 2006 Feb 3;340(1):200-8. doi: 10.1016/j.bbrc.2005.12.001. Epub 2005 Dec 9.
Although processing of the hepatitis C virus (HCV) polyprotein and characterization of each of its viral proteins have been described in detail, analysis of the structure and assembly of HCV particles has been hampered by the lack of a robust cell culture system to support efficient replication of HCV. In this study, we generated HCV-like particles (HCV-LP) using a recombinant baculovirus encoding structural and a part of non-structural proteins in a human hepatoma cell line. The HCV-LP exhibited a buoyant density of 1.17 g/ml in CsCl equilibrium gradient and particles of 40 to 50 nm in diameter. Binding of the HCV-LP to human hepatoma cells was partially inhibited by the treatment with anti-hCD81 antibody, in contrast to the hCD81-independent binding of HCV-LP produced in insect cells. These results indicate that HCV-LP generated in different types of cells exhibit different cellular tropism for binding to target cells.
尽管丙型肝炎病毒（HCV）多聚蛋白的加工过程及其每种病毒蛋白的特性已被详细描述，但由于缺乏支持HCV高效复制的强大细胞培养系统，HCV颗粒的结构和组装分析受到了阻碍。在本研究中，我们在人肝癌细胞系中使用编码结构蛋白和部分非结构蛋白的重组杆状病毒生成了HCV样颗粒（HCV-LP）。HCV-LP在CsCl平衡梯度中的浮力密度为1.17 g/ml，直径为40至50 nm。与昆虫细胞中产生的HCV-LP不依赖hCD81的结合相反，抗hCD81抗体处理可部分抑制HCV-LP与人肝癌细胞的结合。这些结果表明，在不同类型细胞中产生的HCV-LP在与靶细胞结合时表现出不同的细胞嗜性。
