Tunç Ayten Türkkani, Turgut Mehmet, Aslan Hüseyin, Sahin Bünyamin, Yurtseven Mine Ertem, Kaplan Süleyman
Department of Histology and Embryology, Gaziosmanpasa University School of Medicine, Tasliciflik Kampusu TR-60100 Tokat, Turkey.
Brain Res. 2006 Jan 5;1067(1):95-102. doi: 10.1016/j.brainres.2005.10.011. Epub 2005 Dec 19.
Melatonin plays an important role in certain physiological functions and morphological features of various structures. In the current study, the effects of pinealectomy on Purkinje cell number and morphological features of developing cerebellum in the chick were investigated using stereological methods. Fifteen Hybro Broiler newly hatched chicks were divided into three groups: a pinealectomized group (n = 5), sham-operated group (n = 5) and a non-pinealectomized control group (n = 5). Surgical pinealectomy was performed in 3-day-old chicks. In the 8th week, all animals were sacrificed for histopathological evaluation and subsequent stereological analysis. Each layer volume of molecular (+Purkinje cell), granular and white matter in the cerebellum was estimated in all animals. It was found that there was no significant difference for the volume of whole cerebellum and also molecular (+Purkinje cell) layer in these groups (P > 0.05). Nevertheless, the values of granular layer and white matter of sham-operated group were significantly different from those of control and pinealectomized animals (P < 0.01). It was also observed that pinealectomy significantly reduces the Purkinje cell number in cerebellar cortex (P < 0.01). The present study is the first stereological study to demonstrate the histomorphological effects of pinealectomy on the cerebellum in the chick. Our results suggest that pineal gland/melatonin might play an important role in morphological features of the developing cerebellum in the chick.
褪黑素在各种结构的某些生理功能和形态特征中发挥着重要作用。在本研究中，采用体视学方法研究了松果体切除对雏鸡发育中小脑浦肯野细胞数量和形态特征的影响。将15只刚孵化的海波罗肉鸡雏鸡分为三组：松果体切除组（n = 5）、假手术组（n = 5）和非松果体切除对照组（n = 5）。对3日龄雏鸡进行手术切除松果体。在第8周，处死所有动物进行组织病理学评估和随后的体视学分析。估计所有动物小脑分子层（+浦肯野细胞层）、颗粒层和白质的各层体积。结果发现，这些组的整个小脑体积以及分子层（+浦肯野细胞层）均无显著差异（P > 0.05）。然而，假手术组的颗粒层和白质值与对照组和松果体切除动物的显著不同（P < 0.01）。还观察到松果体切除显著减少了小脑皮质中的浦肯野细胞数量（P < 0.01）。本研究是第一项用体视学方法证明松果体切除对雏鸡小脑组织形态学影响的研究。我们的结果表明，松果体/褪黑素可能在雏鸡发育中小脑的形态特征中发挥重要作用。
