Meldrum F C, Heywood B R, Mann S
School of Chemistry, University of Bath, United Kingdom.
Science. 1992 Jul 24;257(5069):522-3. doi: 10.1126/science.1636086.
The iron storage protein ferritin consists of a spherical polypeptide shell (apoferritin) surrounding a 6-nanometer inorganic core of the hydrated iron oxide ferrihydrite (5Fe2O3.9H2O). Previous studies have shown that the in vitro reconstitution of apoferritin yields mineral cores essentially identical to those of the native proteins. A magnetic mineral was synthesized within the nanodimensional cavity of horse spleen ferritin by the use of controlled reconstitution conditions. Transmission electron microscopy and electron diffraction analysis indicate that the entrapped mineral particles are discrete 6-nanometer spherical single crystals of the ferrimagnetic iron oxide magnetite (Fe3O4). The resulting magnetic protein, "magnetoferritin," could have uses in biomedical imaging, cell labeling, and separation procedures.
铁储存蛋白铁蛋白由一个球形多肽壳（脱铁铁蛋白）组成，该壳围绕着一个6纳米的水合氧化铁羟铁矿（5Fe2O3·9H2O）的无机核心。先前的研究表明，脱铁铁蛋白的体外重组产生的矿物核心与天然蛋白质的矿物核心基本相同。通过使用可控的重组条件，在马脾铁蛋白的纳米尺寸腔内合成了一种磁性矿物。透射电子显微镜和电子衍射分析表明，捕获的矿物颗粒是亚铁磁性氧化铁磁铁矿（Fe3O4）的离散6纳米球形单晶。由此产生的磁性蛋白质“磁铁蛋白”可用于生物医学成像、细胞标记和分离程序。
