Yu Jiacheng, Zhang Tongwei, Xu Huangtao, Dong Xiaoli, Cai Yao, Pan Yongxin, Cao Changqian
Biogeomagnetism Group, Paleomagnetism and Geochronology Laboratory, Key Laboratory of Earth and Planetary Physics, Institute of Geology and Geophysics, Chinese Academy of Sciences Beijing 100029 P. R. China
Innovation Academy for Earth Science, CAS Beijing 100029 P. R. China.
RSC Adv. 2019 Nov 29;9(67):39381-39393. doi: 10.1039/c9ra07397c. eCollection 2019 Nov 27.
Thermostable nanoparticles have numerous applications in catalysis and in the oil/gas industry. However, synthesizing these nanoparticles requires expensive polymers. Here, a novel thermostable ferritin named PcFn, originally from the hyperthermophilic archaeon CH1, was overexpressed in , purified and characterized, which could successfully direct the synthesis of thermostable magnetoferritins (M-PcFn) with monodispersed iron oxide nanoparticles in one step. Transmission electron microscopy and magnetic measurements show that the cores of the M-PcFn have an average diameter of 4.7 nm, are well-crystalline and superparamagnetic. Both the PcFn and M-PcFn can resist temperatures up to 110 °C, which is significantly higher than for human H-chain ferritin (HFn) and M-HFn, and comparable to temperatures previously reported for ferritin (PfFn) and M-PfFn. After heating at 110 °C for 30 minutes, PcFn and M-PcFn maintained their secondary structures and PcFn retained 87.4% of its iron uptake activity. This remarkable thermostability of PcFn and M-PcFn suggests potential applications in elevated temperature environments.
热稳定纳米颗粒在催化和石油/天然气工业中有许多应用。然而，合成这些纳米颗粒需要昂贵的聚合物。在此，一种最初来自超嗜热古菌CH1的新型热稳定铁蛋白PcFn在大肠杆菌中过表达、纯化并进行了表征，它能够成功地一步直接合成具有单分散氧化铁纳米颗粒的热稳定磁铁蛋白（M-PcFn）。透射电子显微镜和磁性测量表明，M-PcFn的核心平均直径为4.7 nm，结晶良好且具有超顺磁性。PcFn和M-PcFn都能抵抗高达110°C的温度，这明显高于人H链铁蛋白（HFn）和M-HFn，与先前报道的嗜热栖热菌铁蛋白（PfFn）和M-PfFn的温度相当。在110°C加热30分钟后，PcFn和M-PcFn保持其二级结构，且PcFn保留了87.4%的铁摄取活性。PcFn和M-PcFn这种显著的热稳定性表明它们在高温环境中具有潜在应用。
