谷氨酰胺合成酶在人特发性视网膜前膜中的表达及细胞增殖
Expression of glutamine synthetase and cell proliferation in human idiopathic epiretinal membrane.
作者信息
Kase S, Saito W, Yokoi M, Yoshida K, Furudate N, Muramatsu M, Saito A, Kase M, Ohno S
机构信息
Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Kita-ku, Sapporo 060-8638, Japan.
出版信息
Br J Ophthalmol. 2006 Jan;90(1):96-8. doi: 10.1136/bjo.2005.078394.
BACKGROUND/AIM: The mechanisms of the cellular origin and cell proliferation in the idiopathic epiretinal membrane (ERM) are unsolved. The aim of this study was to examine the expression of cell cycle related molecules and glutamine synthetase (GS), which is expressed in Müller cells and their processes, in ERM tissues.
METHODS
The ERMs were surgically removed using pars plana vitrectomy. Formalin fixed, paraffin embedded ERM tissues were analysed by immunohistochemistry with anti-cyclin D1, p27 (KIP1), proliferating cell nuclear antigen (PCNA), and GS antibodies.
RESULTS
The histopathological findings showed that all the ERMs consisted of oval or spindle mononuclear cells with thin collagen-like tissues. Immunoreactivity for GS was detected in collagen-like tissues of ERM, presenting a continuous, isodense pattern. GS immunopositive cells in all cases expressed PCNA in their nuclei. Nuclear immunoreactivity for cyclin D1 was noted in the ERM constituent cells, whereas p27 (KIP1) positive nuclei were not detected.
CONCLUSION
Cyclin D1 and PCNA were expressed in the idiopathic ERM, which was mainly derived from Müller cells and extensions of their processes.
背景/目的：特发性视网膜前膜（ERM）的细胞起源和细胞增殖机制尚未明确。本研究旨在检测ERM组织中细胞周期相关分子以及在Müller细胞及其突起中表达的谷氨酰胺合成酶（GS）的表达情况。
方法
采用玻璃体切除术手术切除ERM。用抗细胞周期蛋白D1、p27（KIP1）、增殖细胞核抗原（PCNA）和GS抗体对福尔马林固定、石蜡包埋的ERM组织进行免疫组织化学分析。
结果
组织病理学检查结果显示，所有ERM均由椭圆形或梭形单核细胞及薄的胶原样组织构成。在ERM的胶原样组织中检测到GS免疫反应性，呈连续、等密度模式。所有病例中GS免疫阳性细胞的细胞核均表达PCNA。在ERM组成细胞中观察到细胞周期蛋白D1的核免疫反应性，而未检测到p27（KIP1）阳性细胞核。
结论
细胞周期蛋白D1和PCNA在特发性ERM中表达，特发性ERM主要来源于Müller细胞及其突起。
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