Ueki Mari, Morishita Seita, Kohmoto Ryohsuke, Fukumoto Masanori, Suzuki Hiroyuki, Sato Takaki, Kobayashi Takatoshi, Kida Teruyo, Oku Hidehiro, Ikeda Tsunehiko, Shibayama Yuro
Department of Ophthalmology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-City, Osaka, 569-8686, Japan.
Department of Pathology, Osaka Medical College, Takatsuki-City, Osaka, Japan.
Int Ophthalmol. 2016 Oct;36(5):713-8. doi: 10.1007/s10792-016-0194-7. Epub 2016 Feb 8.
To evaluate the histopathological findings of idiopathic and secondary epithelial membranes (ERMs). This study involved 19 ERM cases that underwent pars plana vitrectomy (PPV). ERM specimens were obtained from each patient during PPV and immediately fixed in 10 % formalin. Paraffin sections were stained with hematoxylin eosin (HE) and immunohistochemical analysis was performed with glial fibrillary acidic protein (GFAP), Ki-67, CD34, and nestin antibodies. The 19 ERM cases included 11 idiopathic ERM cases and 8 secondary ERM cases i.e., 2 eyes that underwent PPV for retinal detachment and 6 eyes that underwent PPV for proliferative diabetic retinopathy. HE staining showed that some of the idiopathic ERM specimens consisted of internal limiting membrane. In contrast, numerous invasive cells were observed in the secondary ERM specimens compared to the idiopathic ERM specimens. Immunohistochemical analysis revealed GFAP-positive cells in 4 of the 11 idiopathic ERMs cases, yet no nestin-, Ki-67-, or CD34-positive cells in those cases. In contrast, there were 4 GFAP-positive cases, 2 Ki67-positive cases, 3 CD34-positive cases, and 7 cases including nestin-positive cells. The findings of this study indicate that there are different histological characteristics between idiopathic and secondary ERM and that mature nestin-positive cells in the retina might be related to secondary ERM formation.
评估特发性和继发性视网膜前膜（ERM）的组织病理学特征。本研究纳入了19例行玻璃体切割术（PPV）的ERM病例。在PPV过程中从每位患者获取ERM标本，并立即固定于10%福尔马林中。石蜡切片进行苏木精-伊红（HE）染色，并使用胶质纤维酸性蛋白（GFAP）、Ki-67、CD34和巢蛋白抗体进行免疫组织化学分析。19例ERM病例包括11例特发性ERM病例和8例继发性ERM病例，即2只因视网膜脱离行PPV的眼和6只因增殖性糖尿病视网膜病变行PPV的眼。HE染色显示，部分特发性ERM标本由内界膜组成。相比之下，与特发性ERM标本相比，继发性ERM标本中观察到大量浸润细胞。免疫组织化学分析显示，11例特发性ERM病例中有4例存在GFAP阳性细胞，但这些病例中未发现巢蛋白、Ki-67或CD34阳性细胞。相比之下，继发性ERM病例中有4例GFAP阳性、2例Ki67阳性、3例CD34阳性以及7例存在巢蛋白阳性细胞。本研究结果表明，特发性和继发性ERM之间存在不同的组织学特征，并且视网膜中成熟的巢蛋白阳性细胞可能与继发性ERM的形成有关。
