Núñez María J, Balboa José, Rodrigo Elena, Brenlla Julio, González-Peteiro Mercedes, Freire-Garabal Manuel
Neuroimmunology Laboratory, Department of Pharmacology, School of Medicine, C/San Francisco, s/n, 15782 Santiago de Compostela, A Coruña, Spain.
Neurosci Lett. 2006 Apr 3;396(3):247-51. doi: 10.1016/j.neulet.2005.11.042. Epub 2005 Dec 20.
We studied the effects of fluoxetine, a non-tricyclic antidepressant drug that selectively inhibits re-uptake of serotonin by presinaptic neurons in the brain, on cellular immune responses in mice exposed to a chronic auditory stressor. The natural killer (NK) cell activity was reduced after 4, 8, 12, 16 and 20 days of stress exposure with a partial recovery on days 16 and 20. Daily treatment with fluoxetine partially reversed these adverse effects of stress in a dose-dependent manner. Significant differences appeared when fluoxetine was administered at 2 mg/kg and maximum effect was reached at doses of 5 mg/kg. The capacity of T cells to generate cytotoxic T-lymphocytes (CTL) in mixed lymphocyte cultures and in vivo was reduced after 4 days of stress application and this effect was partially reduced when mice were injected with 5 mg/kg of fluoxetine. Nevertheless, in our experiments, fluoxetine did not significantly affect the cellular immunity in unstressed mice. In conclusion, fluoxetine seems to partially recover the adverse effects of chronic stress on cellular immune response.
我们研究了氟西汀（一种非三环类抗抑郁药，可选择性抑制大脑中突触前神经元对血清素的再摄取）对暴露于慢性听觉应激源的小鼠细胞免疫反应的影响。在应激暴露4、8、12、16和20天后，自然杀伤（NK）细胞活性降低，在第16天和第20天有部分恢复。每日用氟西汀治疗以剂量依赖方式部分逆转了应激的这些不利影响。当以2mg/kg给予氟西汀时出现显著差异，在5mg/kg剂量时达到最大效果。在混合淋巴细胞培养物中和体内，施加应激4天后T细胞产生细胞毒性T淋巴细胞（CTL）的能力降低，当给小鼠注射5mg/kg氟西汀时，这种效应部分降低。然而，在我们的实验中，氟西汀对未受应激的小鼠的细胞免疫没有显著影响。总之，氟西汀似乎部分恢复了慢性应激对细胞免疫反应的不利影响。
