高效液相色谱法同时测定大鼠血浆中的阿莫西林和雷尼替丁
Simultaneous determination of amoxicillin and ranitidine in rat plasma by high-performance liquid chromatography.
作者信息
Li Wei, Tan Fengping, Zhao Kang
机构信息
The College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin, PR China.
出版信息
J Pharm Biomed Anal. 2006 May 3;41(2):594-8. doi: 10.1016/j.jpba.2005.11.024. Epub 2005 Dec 20.
A high-performance liquid chromatography method using ultraviolet detection at 230 nm for the simultaneous determination of amoxicillin and ranitidine in rat plasma has been validated. Plasma samples after pretreatment with acetonitrile to effect deproteinization were dried under N2 and reconstituted with water. The standard calibration curves for amoxicillin and ranitidine were linear (r2=0.9999) over the concentration range of 0.2-20 microg ml-1 and 0.03-6 microg ml-1 in rat plasma, respectively. The intra- and inter-day assay variability range for amoxicillin was 2.4-8.5% and 3.2-11.7%, and for ranitidine was 1.7-9.0% and 4.5-10.1%, respectively. This method has been successfully applied to a pharmacokinetic study after oral coadministration of amoxicillin and ranitidine to rats.
已验证一种使用230nm紫外检测的高效液相色谱法，用于同时测定大鼠血浆中的阿莫西林和雷尼替丁。用乙腈进行预处理以实现蛋白质沉淀后的血浆样品在N2下干燥，并用重水复溶。阿莫西林和雷尼替丁的标准校准曲线在大鼠血浆中分别在0.2 - 20μg ml-1和0.03 - 6μg ml-1的浓度范围内呈线性（r2 = 0.9999）。阿莫西林的日内和日间测定变异范围分别为2.4 - 8.5%和3.2 - 11.7%，雷尼替丁的分别为1.7 - 9.0%和4.5 - 10.1%。该方法已成功应用于大鼠口服阿莫西林和雷尼替丁联合给药后的药代动力学研究。
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