Li Yun-Xia, Jiang Xue-Hua, Huang Liang, Lu Jing, Zhou Jing
Department of Clinical Pharmacy, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
J Pharm Biomed Anal. 2006 May 3;41(2):628-32. doi: 10.1016/j.jpba.2005.12.006. Epub 2006 Jan 18.
A simple high-performance liquid chromatographic method was developed to study the pharmacokinetics of phillyrin in rat after intravenous administration. Plasma was extracted with ethyl acetate after addition of the internal standard, arctiin. Separation was achieved on a reversed-phase C18 column with UV detection at 228 nm. The calibration curves were linear ranging from 0.052 to 26.670 microg/ml. The intra- and inter-day precisions were no more than 9.83% and 12.31%, respectively. The average recovery of phillyrin was 95.44% from plasma. And the limit of quantification (LOQ) was estimated as 0.026 microg/ml with an intra-day relative standard deviation (R.S.D.)<or=20%. The analytical sensitivity and accuracy of this assay were adequate for characterization of phillyrin in rat plasma and the assay has been applied successfully to the in vivo kinetic study of phillyrin in rats.
建立了一种简单的高效液相色谱法，用于研究大鼠静脉注射连翘苷后的药代动力学。加入内标物牛蒡子苷后，用乙酸乙酯萃取血浆。采用反相C18柱，在228nm处进行紫外检测实现分离。校准曲线在0.052至26.670μg/ml范围内呈线性。日内和日间精密度分别不超过9.83%和12.31%。连翘苷从血浆中的平均回收率为95.44%。定量限（LOQ）估计为0.026μg/ml，日内相对标准偏差（R.S.D.）≤20%。该分析方法的灵敏度和准确性足以用于大鼠血浆中连翘苷的表征，且已成功应用于连翘苷在大鼠体内的动力学研究。
