用于支持药物研发的溶解度测定的化学发光氮检测器的评估。
Evaluation of the chemiluminescent nitrogen detector for solubility determinations to support drug discovery.
作者信息
Bhattachar Shobha N, Wesley James A, Seadeek Christopher
机构信息
Pfizer Global Research and Development, Ann Arbor Laboratories, 2800 Plymouth Road, Ann Arbor, MI 48105, USA.
出版信息
J Pharm Biomed Anal. 2006 Apr 11;41(1):152-7. doi: 10.1016/j.jpba.2005.11.009. Epub 2005 Dec 20.
Solubility measurements using chemiluminescent nitrogen detection (CLND) has advantages of reduced compound requirement and increased throughput compared to UV-spectrophotometric and HPLC-based measurements. CLND with direct flow injection was evaluated for the measurement of thermodynamic solubility to support drug discovery. The limit of quantitation (LOQ), accuracy, and day-to-day reproducibility of the detector were measured. Measurements made on CLND were compared to those obtained from UV spectrophotometry and HPLC. Based on the results obtained, it was concluded that the CLND performs satisfactorily for discovery stage thermodynamic solubility measurements.
与基于紫外分光光度法和高效液相色谱法的测量相比，使用化学发光氮检测（CLND）进行溶解度测量具有所需化合物量减少和通量增加的优点。对采用直接流动注射的CLND进行了评估，以测量热力学溶解度，为药物研发提供支持。测定了该检测器的定量限（LOQ）、准确度和日常重现性。将CLND的测量结果与紫外分光光度法和高效液相色谱法的测量结果进行了比较。根据所得结果得出结论，CLND在发现阶段的热力学溶解度测量中表现令人满意。
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