Bala I, Bhardwaj V, Hariharan S, Kumar M N V Ravi
Department of pharmaceutics, National Institute of Pharmaceutical Education and Research, NIPER, Sector 67, SAS Nagar, Phase X Mohali 160062, Punjab, India.
J Pharm Biomed Anal. 2006 Jan 23;40(1):206-10. doi: 10.1016/j.jpba.2005.07.006. Epub 2005 Aug 18.
Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene glycol (PEG) column with mobile phase composed of acetonitrile and 5 mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1 microg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively.
开发了用于定量测定抗氧化剂鞣花酸（EA）的紫外（UV）分光光度法和高效液相色谱法。对这些分析方法进行了线性、准确度、日内和日间变异性以及精密度的验证。使用聚乙二醇（PEG）柱洗脱EA，流动相由乙腈和5 mM磷酸二氢钾缓冲液pH 2.5（80:20，v/v）组成。UV法和高效液相色谱（HPLC）法的检测限分别较低，为0.2和0.1微克/毫升。由于对植物化学物质的药学兴趣增加以及它们的溶解性问题，还开展了EA的溶解度研究。筛选了各种有机溶剂和表面活性剂以评估EA的溶解度，发现该化合物可溶于一些药学上可接受的溶剂，如三乙醇胺、聚乙二醇400和N-甲基吡咯烷酮（NMP）。分别使用UV法和HPLC法测定了EA的水相溶解度和pH依赖性溶解度。
