从日本冷却塔分离出的嗜肺军团菌血清1型菌株形成一个独特的基因簇。
Legionella pneumophila serogroup 1 isolates from cooling towers in Japan form a distinct genetic cluster.
作者信息
Amemura-Maekawa Junko, Kura Fumiaki, Chang Bin, Watanabe Haruo
机构信息
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
出版信息
Microbiol Immunol. 2005;49(12):1027-33. doi: 10.1111/j.1348-0421.2005.tb03699.x.
Thirty-one epidemiologically unrelated Legionella pneumophila serogroup 1 isolates (10 from cooling towers, 10 from public spas and/or hot spring baths, and 11 from patients) were analyzed by pulsed-field gel electrophoresis (PFGE) and sequence-based typing (SBT) using 6 loci, flaA, pilE, asd, mip, mompS, and proA. The results of PFGE and SBT analysis indicated that all 10 isolates from cooling towers clustered into a unique type, which was distinct from strains of other environmental sources.
通过脉冲场凝胶电泳（PFGE）和基于序列的分型（SBT）方法，使用6个基因座（flaA、pilE、asd、mip、mompS和proA）对31株流行病学上无关联的嗜肺军团菌血清1型分离株进行了分析。这些分离株分别来自冷却塔（10株）、公共温泉浴场和/或温泉池（10株）以及患者（11株）。PFGE和SBT分析结果表明，所有来自冷却塔的10株分离株聚为一种独特类型，与其他环境来源的菌株不同。
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