Drenning S D, Stout J E, Joly J R, Yu V L
Department of Medicine, University of Pittsburgh School of Medicine, and Veterans Administration Pittsburgh Healthcare System, University Drive C, PA 15240, USA.
J Infect Dis. 2001 Feb 15;183(4):628-32. doi: 10.1086/318525. Epub 2001 Jan 11.
Phenotypic and genotypic methods identify subtypes of Legionella pneumophila, serogroup 1, and match patient and environmental isolates from suspected sources. The strength of this association is limited by the lack of information regarding the frequency and distribution of isolates belonging to various subtypes. In this study, 62 clinical isolates of L. pneumophila, serogroup 1, were subtyped by using pulsed-field gel electrophoresis (PFGE), to determine the distribution and degree of diversity of PFGE patterns among monoclonal antibody (MAb) subtypes. Unexpectedly, 8 of 21 MAb Philadelphia 1 isolates had a common PFGE pattern, and, among 12 MAb OLDA isolates, only 2 PFGE patterns were seen. Our hypothesis was that PFGE patterns were distributed randomly; however, statistical analysis showed that the distribution of subtypes was not random (Fisher's exact test 0.13; P>.05). In light of these results, researchers who do epidemiological investigations should use caution when interpreting the significance of matching PFGE patterns of L. pneumophila, serogroup 1.
表型和基因型方法可鉴定嗜肺军团菌1血清型的亚型，并将患者和疑似来源的环境分离株进行匹配。由于缺乏关于各种亚型分离株的频率和分布的信息，这种关联的强度受到限制。在本研究中，使用脉冲场凝胶电泳（PFGE）对62株嗜肺军团菌1血清型临床分离株进行亚型分析，以确定单克隆抗体（MAb）亚型中PFGE图谱的分布和多样性程度。出乎意料的是，21株MAb费城1型分离株中有8株具有共同的PFGE图谱，在12株MAb OLDA分离株中，仅观察到2种PFGE图谱。我们的假设是PFGE图谱是随机分布的；然而，统计分析表明亚型分布并非随机（Fisher精确检验0.13；P>0.05）。鉴于这些结果，进行流行病学调查的研究人员在解释嗜肺军团菌1血清型匹配PFGE图谱的意义时应谨慎。
