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疟疾微观检测方法与血液学变化的比较研究

Comparative study of microscopic detection methods and haematological changes in malaria.

作者信息

Jain Meenal, Kaur Manmohan

机构信息

Department of Pathology, Sri Aurobindo Institute of Medical Sciences and Medical College, Indore and Gajra Raja Medical College, Gwalior.

出版信息

Indian J Pathol Microbiol. 2005 Oct;48(4):464-7.

Abstract

Quantitative buffy coat (QBC) technique, thick smears, thin smears and conventional buffy coat smears were compared for malarial parasite detection. Of 200 blood samples studied, 70 (35%) samples were positive by QBC technique, 62 (31%) samples by thick smears, 50 (25%) samples by thin smears and only 34 (17%) samples were positive by conventional buffy coat technique. 8 (4%) samples detected by QBC technique alone, had low parasitic index. Haematological analysis was performed on samples positive for malarial parasite. Anaemia was present in 66 (94.28%) samples of which 37 (56.06%) were Plasmodium falciparum, 21 (31.81%) were Plasmodium vivax and 8 (12.12%) had mixed infection (Plasmodium falciparum and Plasmodium vivax). 35 (50%) cases showed normocytic normochromic anaemia. Majority of the samples showed normal total and differential leukocyte count. Thrombocytopenia was found in 49 (70%) samples of which 33 (67.34%) were Plasmodium falciparum.

摘要

对定量血沉棕黄层(QBC)技术、厚涂片、薄涂片和传统血沉棕黄层涂片进行疟疾寄生虫检测比较。在研究的200份血样中,QBC技术检测出70份(35%)样本呈阳性,厚涂片检测出62份(31%)样本呈阳性,薄涂片检测出50份(25%)样本呈阳性,而传统血沉棕黄层技术仅检测出34份(17%)样本呈阳性。仅通过QBC技术检测出的8份(4%)样本,其寄生虫指数较低。对疟疾寄生虫呈阳性的样本进行血液学分析。66份(94.28%)样本存在贫血,其中37份(56.06%)为恶性疟原虫,21份(31.81%)为间日疟原虫,8份(12.12%)为混合感染(恶性疟原虫和间日疟原虫)。35例(50%)表现为正细胞正色素性贫血。大多数样本的白细胞总数和分类计数正常。49份(70%)样本发现血小板减少,其中33份(67.34%)为恶性疟原虫。

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