Don Trong-Ming, Chen Ching-Chung, Lee Chih-Kang, Cheng Wei-Yuan, Cheng Liao-Ping
Department of Chemical and Materials Engineering, Tamkang University, Taipei, Taiwan, ROC 25137.
J Biomater Sci Polym Ed. 2005;16(12):1503-19. doi: 10.1163/156856205774576718.
Chitosan/poly(acrylic acid)/poly(ethylene glycol) diacrylate (PEGDA) composite membranes with a bi-layer configuration were prepared and their potential application as an antibacterial material was examined. A two-step process was adopted. A dope consisting of PEGDA, acrylic acid (AA) and a photoinitiator was cast and then UV-cured on a glass substrate to form a mechanically strong, dense membrane. Subsequently, the membrane was coated with a layer of solution composed of chitosan, AA and water. As the majority of AA diffused downwards into the supporting layer underneath, chitosan coagulated with residual AA to form a nano-layer on the top surface by means of UV irradiation. Low-voltage field-emission scanning electron microscopy was used to observe the membrane morphology and to measure the thickness of the top layer. Contact angle measurements indicated a top layer mixed with chitosan and poly(acrylic acid), which was confirmed by chemical composition analysis of X-ray photon spectroscopy. The antibacterial activities of the formed membranes were tested both with respect to a Gram-negative (Escherichia coli) and a Gram-positive (Staphylococcus aureus) bacteria.
