Sparnacci Katia, Laus Michele, Tondelli Luisa, Bernardi Cinzia, Magnani Laura, Corticelli Franco, Marchisio Marco, Ensoli Barbara, Castaldello Arianna, Caputo Antonella
Department of Environmental and Life Sciences INSTM, University of Piemonte Orientale, Spalto Marengo 33, 15100 Alessandria, Italy.
J Biomater Sci Polym Ed. 2005;16(12):1557-74. doi: 10.1163/156856205774576673.
Functional poly(methyl methacrylate) core-shell microspheres were prepared by dispersion polymerization. An appropriate selection of experimental parameters and in particular of the initiator and stabilizer amount and of the medium solvency power allowed a monodisperse sample as large as 600 nm to be prepared. To this purpose, low initiator concentration, high steric stabilizer amount and a low solvency power medium were employed. The microspheres present a core-shell structure in which the outer shell is constituted by the steric stabilizer which affords carboxylic groups able to interact with basic proteins, such as trypsin, whose adsorption is essentially driven by the carboxylic group density in the microsphere shell. Finally, fluorescent microspheres were prepared for biodistribution studies and shown to be readily taken up by the cells both in vitro and in vivo. These results suggest that these microspheres are promising delivery systems for the development of novel protein-based vaccines.
通过分散聚合制备了功能性聚甲基丙烯酸甲酯核壳微球。适当选择实验参数，特别是引发剂和稳定剂的用量以及介质的溶解能力，可制备出直径达600 nm的单分散样品。为此，采用了低引发剂浓度、高空间稳定剂用量和低溶解能力的介质。微球呈现核壳结构，其中外壳由空间稳定剂构成，该稳定剂提供能够与碱性蛋白质（如胰蛋白酶）相互作用的羧基，其吸附主要由微球壳中的羧基密度驱动。最后，制备了用于生物分布研究的荧光微球，并证明其在体外和体内均能被细胞轻易摄取。这些结果表明，这些微球是开发新型蛋白质疫苗的有前景的递送系统。
