智能水凝胶颗粒：生物标志物捕获：一步法亲和纯化、尺寸排阻及抗降解保护。
Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.
作者信息
Luchini Alessandra, Geho David H, Bishop Barney, Tran Duy, Xia Cassandra, Dufour Robert L, Jones Clinton D, Espina Virginia, Patanarut Alexis, Zhou Weidong, Ross Mark M, Tessitore Alessandra, Petricoin Emanuel F, Liotta Lance A
机构信息
CRO-IRCCS National Cancer Institute, Aviano, Italy.
出版信息
Nano Lett. 2008 Jan;8(1):350-61. doi: 10.1021/nl072174l. Epub 2007 Dec 13.
Abstract
Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.
摘要
疾病相关的血液生物标志物存在于白蛋白等高丰度蛋白质的复杂混合物中，其浓度极低。我们已将一种亲和诱饵分子引入N-异丙基丙烯酰胺中，以制备一种颗粒，该颗粒能在溶液中一步在几分钟内执行三种独立功能：（a）分子大小筛分，（b）亲和捕获所有溶液相目标分子，以及（c）完全保护收获的蛋白质不被酶降解。捕获的分析物可很容易地进行电洗脱以用于分析。
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