Monteiro E, Varzim G, Silva R, da Costa Barreira, Lopes C
Portuguese Institute of Oncology, CROP, SA-Porto, Molecular Oncology Unit, Rua António Bernardino Almeida, 4200-072 Porto, Portugal.
Rev Laryngol Otol Rhinol (Bord). 2005;126(3):135-40.
The human OGG1 (hOGG1) gene encodes a DNA glycosylase involved in the excision repair of 8-hydroxy-2'-deoxyguanine (8-OH-dG) from oxidatively-damaged DNA. Ser326Cys polymorphism in the hOGG1 gene is involved in the repair of 8-hydroxyguanine in oxidatively damaged DNA, and appears to be related to susceptibility to certain smoking and alcohol-related orolaryngeal cancers.
To analyse if hOGG1 Ser326Cys (exon 7: m6) polymorphism is associated with tumour localization, T, stage and histologic differentiation, and if radiotherapy results were influenced by this polymorphism.
Blood samples were obtained before treatment from seventy one patients with laryngeal cancer and screened by a PCR-RFLP method.
Although hOGG1 gene is important in DNA repair mechanisms, no significant association was observed between hOGG1 Ser326Cys (exon 7: m6) polymorphism, tumour characteristics and radiotherapy results.
So the analysis of this polymorphism is not important for treatment decision in laryngeal cancer patients.
人类OGG1（hOGG1）基因编码一种DNA糖基化酶，参与从氧化损伤的DNA中切除8-羟基-2'-脱氧鸟嘌呤（8-OH-dG）。hOGG1基因中的Ser326Cys多态性参与氧化损伤DNA中8-羟基鸟嘌呤的修复，并且似乎与某些吸烟和酒精相关的口腔咽喉癌易感性有关。
分析hOGG1 Ser326Cys（外显子7：m6）多态性是否与肿瘤定位、T分期、阶段和组织学分化相关，以及这种多态性是否影响放疗结果。
在治疗前从71例喉癌患者中采集血样，并通过PCR-RFLP方法进行筛查。
尽管hOGG1基因在DNA修复机制中很重要，但未观察到hOGG1 Ser326Cys（外显子7：m6）多态性、肿瘤特征和放疗结果之间存在显著关联。
因此，对这种多态性的分析对喉癌患者的治疗决策并不重要。
