一种基于适体的无试剂电子传感器的信号开启架构，通过靶标诱导的链置换实现。
A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement.
作者信息
Xiao Yi, Piorek Brian D, Plaxco Kevin W, Heeger Alan J
机构信息
Department of Physics, Institute for Polymers and Organic Solids, Program in BioMolecular Science and Engineering, University of California, Santa Barbara, California 93106, USA.
出版信息
J Am Chem Soc. 2005 Dec 28;127(51):17990-1. doi: 10.1021/ja056555h.
Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as approximately 3 nM.
凝血酶结合使适配体的交替G-四链体构象稳定，释放出甲基蓝（MB）标记的寡核苷酸，产生一个灵活的单链DNA元件。这使得MB标签能够与金电极表面碰撞，在低至约3 nM的凝血酶浓度下产生易于检测的法拉第电流。
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