用于小分子的无标记且无需试剂的基于适配体的传感器。
Label-free and reagentless aptamer-based sensors for small molecules.
作者信息
Zayats Maya, Huang Ye, Gill Ron, Ma Chun-an, Willner Itamar
机构信息
The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
出版信息
J Am Chem Soc. 2006 Oct 25;128(42):13666-7. doi: 10.1021/ja0651456.
A label free, reagentless aptasensor for adenosine is developed on an ISFET device. The separation of an aptamer/nucleic acid duplex by adenosine leads to the aptamer/adenosine complex that alters the gate potential of the ISFET. The sensitivity limit of the device is 5 x 10-5 M. Also, the immobilization of the aptamer/nucleic acid duplex on an Au-electrode and the separation of the duplex by adenosine mono-phosphate (AMP) enable the electrochemical detection of adenosine by faradaic impedance spectroscopy. The separation of the aptamer/nucleic acid duplex by adenosine and the formation of the aptamer/adenosine complex results in a decrease in the interfacial electron-transfer resistance in the presence of [Fe(CN)6]3-/4- as redox active substrate.
一种用于腺苷的无标记、无需试剂的适体传感器在离子敏感场效应晶体管（ISFET）器件上研制成功。腺苷使适体/核酸双链体分离，形成适体/腺苷复合物，从而改变ISFET的栅极电位。该器件的灵敏度极限为5×10⁻⁵ M。此外，适体/核酸双链体固定在金电极上，且单磷酸腺苷（AMP）能使双链体分离，这使得通过法拉第阻抗谱对腺苷进行电化学检测成为可能。在存在[Fe(CN)₆]³⁻/⁴⁻作为氧化还原活性底物的情况下，腺苷使适体/核酸双链体分离并形成适体/腺苷复合物，导致界面电子转移电阻降低。
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