Balkwill D L, Labeda D P, Casida L E
Can J Microbiol. 1975 Mar;21(3):252-62. doi: 10.1139/m75-036.
A simplified procedure is presented for releasing and concentrating indigenous microbial cells from soil for viewing by transmission electron microscopy as thin sections or replicas of frozen-etched preparations. This procedure is compared with two others reported earlier, and their relative merits are discussed as concerns the choice of procedure for the cellular information desired from the soil. Freeze-etching showed that the cell types and size distributions for cells which have been released and concentrated from soil are in general agreement with those for cells in a crude soil slurry in which no attempt to release and concentrate cells was made. Microcolonies were present both in the crude slurry and in the discard soil debris centrifugation pellets from the cell release and concentration procedures. In contrast to the historic assumptions, these microcolonies, as well as some individual cells embedded in soil debris could not be broken up and (or) dislodged so that they would be washed from the soil. The relative numbers of these cells remaining with the soil debris, however, could not be quantitated in the present study.
本文介绍了一种简化程序，用于从土壤中释放和浓缩本地微生物细胞，以便通过透射电子显微镜观察薄切片或冷冻蚀刻制剂的复制品。将该程序与之前报道的另外两种程序进行了比较，并讨论了它们在根据所需土壤细胞信息选择程序方面的相对优点。冷冻蚀刻表明，从土壤中释放和浓缩的细胞的细胞类型和大小分布与未尝试释放和浓缩细胞的粗土壤浆液中的细胞总体一致。在粗浆液以及细胞释放和浓缩程序的废弃土壤碎片离心沉淀中均存在微菌落。与历史假设相反，这些微菌落以及一些嵌入土壤碎片中的单个细胞无法被分解和（或）去除，以便从土壤中冲洗掉。然而，在本研究中无法对这些残留在土壤碎片中的细胞的相对数量进行定量。
