Flessner M F, Dedrick R L, Reynolds J C
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
Am J Physiol. 1992 Jul;263(1 Pt 2):F15-23. doi: 10.1152/ajprenal.1992.263.1.F15.
Protein transport occurs between the blood and the peritoneal cavity during clinical procedures, but events within the surrounding tissue space are poorly understood. We used quantitative autoradiography to examine the tissue concentration profiles of immunoglobulin G (IgG) in regions surrounding the peritoneal cavity. We have varied the route of administration (intravenous or intraperitoneal), the osmolality of the dialysis solution (isotonic or hypertonic), and the time of analysis (20 or 200 min). After intravenous injection, IgG profiles were relatively flat in most tissues and were not affected by time or osmolality. Concentrations corresponded to the capillary density in specific tissues. After intraperitoneal administration, the IgG tissue profiles were significantly steeper than after intravenous administration. The tissue concentrations increased with time but decreased when a hypertonic solution was substituted for an isotonic solution. Hypertonic dialysis causes a water flux into the cavity, which dilutes the contents but does not prevent penetration of protein into the surrounding tissue. Based on IgG movement in tissue during hypertonic dialysis, the peritoneum appears to function as a heterogeneous structure, which allows osmotically induced water transport into the cavity in some regions with simultaneous transport of hydrostatic pressure-driven water and solute flow from the cavity into the tissue in other regions.
在临床操作过程中，蛋白质在血液和腹腔之间进行转运，但对周围组织间隙内的情况却知之甚少。我们采用定量放射自显影技术来检测腹腔周围区域免疫球蛋白G（IgG）的组织浓度分布。我们改变了给药途径（静脉注射或腹腔内注射）、透析液的渗透压（等渗或高渗）以及分析时间（20分钟或200分钟）。静脉注射后，大多数组织中的IgG分布相对平缓，且不受时间或渗透压的影响。浓度与特定组织中的毛细血管密度相对应。腹腔内给药后，IgG的组织分布曲线比静脉注射后明显更陡。组织浓度随时间增加，但当用高渗溶液替代等渗溶液时则降低。高渗透析会使水流入腹腔，这会稀释腹腔内容物，但并不能阻止蛋白质渗透到周围组织中。基于高渗透析过程中IgG在组织中的移动情况，腹膜似乎起到了异质结构的作用，它在一些区域允许渗透压诱导的水转运进入腹腔，同时在其他区域使静水压力驱动的水和溶质从腹腔流入组织。
