Ménan Hervé, Banzouzi Jean-Théophile, Hocquette Antoine, Pélissier Yves, Blache Yves, Koné Moussa, Mallié Michèle, Assi Laurent Aké, Valentin Alexis
Laboratoire d'Immunologie et Parasitologie, EA 2413, UFR des Sciences Pharmaceutiques et Biologiques, 15, Av Ch. Flahault, BP 14491, 34093 Montpellier cedex 2, France.
J Ethnopharmacol. 2006 Apr 21;105(1-2):131-6. doi: 10.1016/j.jep.2005.10.027. Epub 2005 Dec 20.
Eighteen plants originating from Ivory Coast were selected by ethnobotanical survey as plants commonly used by traditional healers for the treatment of malaria. Extracts of these plants were tested on two strains of Plasmodium falciparum: FcM29-Cameroon (chloroquine-resistant strain) and a Nigerian chloroquine-sensitive strain. The powdered plants were used to prepare three kinds of extracts: by decoction in water, in ethanol (95%) and in pentane. A radioactive micromethod allowed the evaluation of the antiplasmodial in vitro activity of the extracts on P. falciparum. Concentrations inhibiting 50% of the parasite growth (IC50) ranged from 18 microg/ml to more than 500 microg/ml for aqueous and ethanol extracts and from 4.3 microg/ml to more than 500 microg/ml for pentane extracts. Cytotoxicity was estimated on A375 melanoma cells and a cytotoxicity/antiplasmodial index (CAR) was calculated for each extract, ranging from 1 to 10. The pentane extracts of Cola caricaefolia and Uvaria afzelii, which revealed the strongest antiplasmodial activity had CAR values of about 10.
通过民族植物学调查，从科特迪瓦采集的18种植物被选出来，这些植物是传统治疗师常用于治疗疟疾的植物。这些植物的提取物在两种恶性疟原虫菌株上进行了测试：FcM29-喀麦隆（氯喹抗性菌株）和一种尼日利亚氯喹敏感菌株。将植物粉末用于制备三种提取物：用水煎煮、用乙醇（95%）和戊烷提取。一种放射性微量法可用于评估提取物对恶性疟原虫的体外抗疟活性。水提取物和乙醇提取物抑制50%寄生虫生长的浓度（IC50）范围为18微克/毫升至超过500微克/毫升，戊烷提取物的范围为4.3微克/毫升至超过500微克/毫升。在A375黑色素瘤细胞上评估细胞毒性，并为每种提取物计算细胞毒性/抗疟指数（CAR），范围为1至10。显示出最强抗疟活性的可乐果叶和乌瓦利亚阿费利的戊烷提取物的CAR值约为10。
