DNA臂负责具体工作以确保λ位点特异性重组的方向性。
DNA arms do the legwork to ensure the directionality of lambda site-specific recombination.
作者信息
Radman-Livaja Marta, Biswas Tapan, Ellenberger Tom, Landy Arthur, Aihara Hideki
机构信息
Division of Biology and Medicine-Box G, Brown University, Providence, RI 02912, USA.
出版信息
Curr Opin Struct Biol. 2006 Feb;16(1):42-50. doi: 10.1016/j.sbi.2005.12.003. Epub 2005 Dec 20.
Abstract
The integrase protein of bacteriophage lambda (Int) catalyzes site-specific recombination between lambda phage and Escherichia coli genomes. Int is a tyrosine recombinase that binds to DNA core sites via a C-terminal catalytic domain and to a collection of arm DNA sites, distant from the site of recombination, via its N-terminal domain. The arm sites, in conjunction with accessory DNA-bending proteins, provide a means of regulating the efficiency and directionality of Int-catalyzed recombination. Recent crystal structures of lambda Int tetramers bound to synaptic and Holliday junction intermediates, together with new biochemical data, suggest a mechanism for the allosteric control of the recombination reaction through arm DNA binding interactions.
摘要
噬菌体λ的整合酶蛋白（Int）催化λ噬菌体与大肠杆菌基因组之间的位点特异性重组。Int是一种酪氨酸重组酶，它通过C端催化结构域与DNA核心位点结合，并通过其N端结构域与远离重组位点的一系列臂DNA位点结合。臂位点与辅助DNA弯曲蛋白一起，提供了一种调节Int催化重组效率和方向性的方式。最近与突触和霍利迪连接中间体结合的λ Int四聚体的晶体结构，以及新的生化数据，提示了一种通过臂DNA结合相互作用对重组反应进行变构控制的机制。
相似文献
1
DNA arms do the legwork to ensure the directionality of lambda site-specific recombination.DNA臂负责具体工作以确保λ位点特异性重组的方向性。
Curr Opin Struct Biol. 2006 Feb;16(1):42-50. doi: 10.1016/j.sbi.2005.12.003. Epub 2005 Dec 20.
2
Mapping the λ Integrase bridges in the nucleoprotein Holliday junction intermediates of viral integrative and excisive recombination.绘制λ整合酶在病毒整合和切除重组的核蛋白霍利迪连接中间体中的桥梁。
Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12366-71. doi: 10.1073/pnas.1413007111. Epub 2014 Aug 11.
3
Differential affinity and cooperativity functions of the amino-terminal 70 residues of lambda integrase.λ整合酶氨基末端70个残基的差异亲和力和协同功能。
J Mol Biol. 2002 Dec 6;324(4):775-89. doi: 10.1016/s0022-2836(02)01199-3.
4
Amino-terminal domain interactions of lambda integrase on arm-type DNA.λ整合酶在臂型DNA上的氨基末端结构域相互作用
Biochem Biophys Res Commun. 2008 Nov 7;376(1):139-42. doi: 10.1016/j.bbrc.2008.08.109. Epub 2008 Aug 31.
5
Lambda integrase: armed for recombination.λ整合酶：为重组做好准备。
Curr Biol. 2005 Sep 6;15(17):R658-60. doi: 10.1016/j.cub.2005.08.031.
6
The amino terminus of bacteriophage lambda integrase is involved in protein-protein interactions during recombination.噬菌体λ整合酶的氨基末端在重组过程中参与蛋白质-蛋白质相互作用。
J Bacteriol. 2000 Feb;182(4):1024-34. doi: 10.1128/JB.182.4.1024-1034.2000.
7
Interactions between lambda Int molecules bound to sites in the region of strand exchange are required for efficient Holliday junction resolution.与链交换区域位点结合的λ Int 分子之间的相互作用是高效霍利迪连接点拆分所必需的。
J Mol Biol. 1990 Oct 20;215(4):523-35. doi: 10.1016/s0022-2836(05)80165-2.
8
A biotin interference assay highlights two different asymmetric interaction profiles for lambda integrase arm-type binding sites in integrative versus excisive recombination.生物素干扰分析揭示了λ整合酶臂型结合位点在整合与切除重组中两种不同的不对称相互作用模式。
J Biol Chem. 2008 May 2;283(18):12402-14. doi: 10.1074/jbc.M800544200. Epub 2008 Mar 4.
9
Synaptic intermediates in bacteriophage lambda site-specific recombination: integrase can align pairs of attachment sites.噬菌体λ位点特异性重组中的突触中间体：整合酶可使附着位点对排列对齐。
EMBO J. 1993 Dec;12(12):4567-76. doi: 10.1002/j.1460-2075.1993.tb06145.x.
10
Arm site independence of coliphage HK022 integrase in human cells.噬菌体 HK022 整合酶在人细胞中臂位独立性。
Mol Genet Genomics. 2011 May;285(5):403-13. doi: 10.1007/s00438-011-0614-3. Epub 2011 Mar 26.
引用本文的文献
1
Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix-turn-helix protein RdfS.寡聚翼状螺旋-转角-螺旋蛋白RdfS控制整合性接合元件切除和转移的结构基础
Nucleic Acids Res. 2025 Mar 20;53(6). doi: 10.1093/nar/gkaf249.
2
Landscape of mobile genetic elements and their antibiotic resistance cargo in prokaryotic genomes.原核生物基因组中移动遗传元件及其抗生素抗性载物的景观。
Nucleic Acids Res. 2022 Apr 8;50(6):3155-3168. doi: 10.1093/nar/gkac163.
3
Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.序列分析酪氨酸重组酶可对原核基因组中的移动遗传元件进行注释。
Mol Syst Biol. 2021 May;17(5):e9880. doi: 10.15252/msb.20209880.
4
Molecular Cloning Designer Simulator (MCDS): All-in-one molecular cloning and genetic engineering design, simulation and management software for complex synthetic biology and metabolic engineering projects.分子克隆设计模拟器（MCDS）：用于复杂合成生物学和代谢工程项目的一体化分子克隆与基因工程设计、模拟及管理软件。
Metab Eng Commun. 2016 May 12;3:173-186. doi: 10.1016/j.meteno.2016.05.003. eCollection 2016 Dec.
5
Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int.实时单分子束缚粒子运动分析揭示了 Flp 位点特异性重组酶与 Cre 和 λ Int 的机制相似性和差异。
Nucleic Acids Res. 2013 Aug;41(14):7031-47. doi: 10.1093/nar/gkt424. Epub 2013 Jun 3.
6
A new large-DNA-fragment delivery system based on integrase activity from an integrative and conjugative element.一种新型的基于整合和转座元件整合酶活性的大片段 DNA 递送系统。
Appl Environ Microbiol. 2013 Jul;79(14):4440-7. doi: 10.1128/AEM.00711-13. Epub 2013 May 17.
7
A single copy integration vector that integrates at an engineered site on the Staphylococcus aureus chromosome.一种单拷贝整合载体，可整合到金黄色葡萄球菌染色体上的一个工程位点。
BMC Res Notes. 2012 Jan 5;5:5. doi: 10.1186/1756-0500-5-5.
8
Arm site independence of coliphage HK022 integrase in human cells.噬菌体 HK022 整合酶在人细胞中臂位独立性。
Mol Genet Genomics. 2011 May;285(5):403-13. doi: 10.1007/s00438-011-0614-3. Epub 2011 Mar 26.
9
The CTCF insulator protein forms an unusual DNA structure.CTCF 绝缘子蛋白形成一种不寻常的 DNA 结构。
BMC Mol Biol. 2010 Dec 21;11:101. doi: 10.1186/1471-2199-11-101.
10
Topoisomerases and site-specific recombinases: similarities in structure and mechanism.拓扑异构酶和位点特异性重组酶：结构和机制上的相似性。
Crit Rev Biochem Mol Biol. 2010 Dec;45(6):520-34. doi: 10.3109/10409238.2010.513375.
本文引用的文献
1
A mechanism for gene conversion in fungi.真菌中基因转换的一种机制。
Genet Res. 2007 Dec;89(5-6):285-307. doi: 10.1017/S0016672308009476.
2
Receipt of the C-terminal tail from a neighboring lambda Int protomer allosterically stimulates Holliday junction resolution.来自相邻λ整合酶原体的C末端尾巴的接收会变构刺激霍利迪连接体的拆分。
J Mol Biol. 2005 Sep 2;351(5):948-55. doi: 10.1016/j.jmb.2005.06.077.
3
A structural basis for allosteric control of DNA recombination by lambda integrase.λ整合酶对DNA重组进行变构调控的结构基础。
Nature. 2005 Jun 23;435(7045):1059-66. doi: 10.1038/nature03657.
4
Architecture of recombination intermediates visualized by in-gel FRET of lambda integrase-Holliday junction-arm DNA complexes.通过λ整合酶-霍利迪连接体臂DNA复合物的凝胶内荧光共振能量转移可视化重组中间体的结构
Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):3913-20. doi: 10.1073/pnas.0500844102. Epub 2005 Mar 7.
5
Mutations in the amino-terminal domain of lambda-integrase have differential effects on integrative and excisive recombination.λ整合酶氨基末端结构域中的突变对整合和切除重组具有不同的影响。
Mol Microbiol. 2005 Feb;55(4):1104-12. doi: 10.1111/j.1365-2958.2004.04447.x.
6
Non-equivalent interactions between amino-terminal domains of neighboring lambda integrase protomers direct Holliday junction resolution.相邻λ整合酶原体氨基末端结构域之间的非等效相互作用指导霍利迪连接体的拆分。
J Mol Biol. 2005 Jan 21;345(3):475-85. doi: 10.1016/j.jmb.2004.10.068.
7
The efficiency of mispaired ligations by lambda integrase is extremely sensitive to context.λ整合酶错配连接的效率对环境极为敏感。
J Mol Biol. 2004 Oct 1;342(5):1647-58. doi: 10.1016/j.jmb.2004.08.008.
8
Two structural features of lambda integrase that are critical for DNA cleavage by multimers but not by monomers.λ整合酶的两个结构特征对于多聚体而非单体进行DNA切割至关重要。
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2770-5. doi: 10.1073/pnas.0400135101. Epub 2004 Feb 19.
9
Reversible inhibitors of lambda integrase-mediated recombination efficiently trap Holliday junction intermediates and form the basis of a novel assay for junction resolution.λ整合酶介导的重组的可逆抑制剂能有效捕获霍利迪连接中间体，并构成一种用于连接体解析的新型检测方法的基础。
J Biol Chem. 2004 Jan 30;279(5):3472-83. doi: 10.1074/jbc.M309361200. Epub 2003 Nov 18.
10
The molecular basis of co-operative DNA binding between lambda integrase and excisionase.λ整合酶与切除酶之间协同DNA结合的分子基础。
Mol Microbiol. 2003 Oct;50(1):89-99. doi: 10.1046/j.1365-2958.2003.03687.x.
Zotero 插件