Chen P L, Hutchison D J, Sarkar N H, Kramarsky B, Moore D H
Cancer Res. 1975 Mar;35(3):718-28.
In vitro L1210 (V) cell lines contained abundant intracytoplasmic A-particles, numerous C-type particles, a small number of B-type particles, and occasional intracisternal A-particles. The intracytoplasmic A-particles were incorporated into both spiked (B-type) and smooth-surfaced (C-type) particles formed at the budding site. Both B-and C-type particles also developed by gradual accululation of neucleooid material. The particles, particularly the C-type, exhibited a wide range of densities. The cells showed strong surface immunofluoresence for both murine mammary tumor virus and Gross murine leukemia virus antigens and variable degrees of cytoplasmic immunoflurescence for the protein antigens (p1 to p6) of Rauscher leukemia virus. The cells, the culture supernatant, and the purified virus each gave positive reactions with murine mammary tumor virus and murine leukemia virus antisera by immunodiffusion. The viral particles failed to infect C57BL, C57BL/6 X DBA/2F1 (hereafter called BD2F1), BALB/c, Af,and RIIIf mice. Howver, the cells were highly tumorigenic in BD1F-1 mice, moderately tumorigenic in BALB/c mice, but not tumorigenic in C57BL, Af, and RIIIf mice.
体外培养的L1210 (V)细胞系含有丰富的胞质A型颗粒、大量的C型颗粒、少量的B型颗粒以及偶尔出现的核内池A型颗粒。胞质A型颗粒被整合到在出芽位点形成的带刺（B型）和平滑表面（C型）颗粒中。B型和C型颗粒也通过核仁样物质的逐渐积累而形成。这些颗粒，尤其是C型颗粒，表现出广泛的密度范围。这些细胞对小鼠乳腺肿瘤病毒和格罗斯小鼠白血病病毒抗原均表现出强烈的表面免疫荧光，对劳舍尔白血病病毒的蛋白质抗原（p1至p6）表现出不同程度的胞质免疫荧光。这些细胞、培养上清液和纯化病毒通过免疫扩散与小鼠乳腺肿瘤病毒和小鼠白血病病毒抗血清均产生阳性反应。这些病毒颗粒不能感染C57BL、C57BL/6×DBA/2F1（以下简称BD2F1）、BALB/c、Af和RIIIf小鼠。然而，这些细胞在BD1F-1小鼠中具有高度致瘤性，在BALB/c小鼠中具有中度致瘤性，但在C57BL、Af和RIIIf小鼠中不具有致瘤性。
