用于评估尿路致病性大肠杆菌与终末分化人尿道上皮细胞相互作用的新型三维类器官模型。
Novel three-dimensional organoid model for evaluation of the interaction of uropathogenic Escherichia coli with terminally differentiated human urothelial cells.
作者信息
Smith Yarery C, Grande Kerian K, Rasmussen Susan B, O'Brien Alison D
机构信息
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Rm. B4052, 4301 Jones Bridge Rd., Bethesda, MD 20814-4799, USA.
出版信息
Infect Immun. 2006 Jan;74(1):750-7. doi: 10.1128/IAI.74.1.750-757.2006.
Abstract
Human bladder 5637 cells cultivated under microgravity conditions formed organoids that displayed characteristics of in vivo tissue-specific differentiation. Uropathogenic Escherichia coli (UPEC) strain CP9 colonized and penetrated the organoids and induced alpha-hemolysin-mediated exfoliation of uroepithelial cells. We propose these uro-organoids as models that simulate the interactions between UPEC and terminally differentiated human urothelium.
摘要
在微重力条件下培养的人膀胱5637细胞形成了类器官，这些类器官表现出体内组织特异性分化的特征。尿路致病性大肠杆菌（UPEC）菌株CP9定殖并穿透类器官，并诱导α-溶血素介导的尿路上皮细胞脱落。我们提出将这些尿路上皮类器官作为模拟UPEC与终末分化的人尿路上皮之间相互作用的模型。
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