Brown R C, Kostyu J A, Kilgore A
Cancer Res. 1975 Apr;35(4):1053-7.
The quantity of C-type RNA tumor viruses in homogenate-sonicates of thymus-bone marrow tissues of C57BL/6J and RFM/Un mice 10 days after irradiation (X-rays or gamma-rays)-plus-urethan treatments is no greater than that in thymus-bone marrow homogenates from nontreated control mice. These results indicate that the leukemogenic activity, shown to be present in such thymus-bone marrow homogenates at this time after irradiation-plus-urethan treatment, is not due to change in quantity of C-type viruses as has been proposed. Virus quantity in tissues was evaluated by a new procedure that includes use of a microchamber with the sides situated on rotor radii so as to produce a uniform virus-containing sediment of tissue homogenate-sonicate that is evaluated by electron microscopic examination of this sections cut perpendicular to the membrane surface. Samples containing as little as 105 to 106 viruses can be relatively easily counted. Semipurified or purified viruses can also be counted after mixing with a tissue homogenate-bovine serum albumin diluent.
经X射线或γ射线照射加乌拉坦处理10天后，C57BL/6J和RFM/Un小鼠胸腺-骨髓组织匀浆超声处理物中C型RNA肿瘤病毒的数量不高于未处理对照小鼠胸腺-骨髓匀浆中的数量。这些结果表明，照射加乌拉坦处理后此时此类胸腺-骨髓匀浆中显示出的致白血病活性并非如所提出的那样是由于C型病毒数量的变化。通过一种新方法评估组织中的病毒数量，该方法包括使用一个微室，其侧面位于转子半径上，以便产生组织匀浆超声处理物的均匀含病毒沉淀物，通过对垂直于膜表面切割的切片进行电子显微镜检查来评估该沉淀物。含有低至105至106个病毒的样品相对容易计数。半纯化或纯化的病毒与组织匀浆-牛血清白蛋白稀释剂混合后也可计数。
