Mackey L, Jarrett W, Wilson L
Cancer Res. 1975 Apr;35(4):1064-8.
A mixed-immunoglobulin rosette technique has been developed for the detection of antibodies to feline oncornavirus-associated cell membrane antigens. Lymphoblastoid cells infected with feline leukemia virus were incubated with test sera and then fixed in paraformaldehyde. They were then exposed to a rabbit anti-cat immunoglobulin G serum. Antigen-antibody reactions were detected by mixing the cells with sheep erythrocytes sensitized with cat anti-sheep red blood cell serum; the presence of cat antibody on the lymphoid cells was registered by the formation of sheep red blood cell rosettes around them. The method was shown to be at least 10 times more sensitive than indirect immunofluorescence. A high degree of correlation was shown between the mixed-immunoglobulin rosette and indirect immunofluorescence tests, using both feline leukemia virus-infected cat and dog cells as targets. The results indicate that the tests are likely to be measuring similar reactions. Using the indirect immunofluorescence test we found that 75% of cats with lymphoid neoplasia had no demonstrable antibodies. Twenty-four of such indirect immunofluorescence-negative sera were tested by the mixed-immunoglobulin rosette technique; antibody was shown in at least seven of these sera.
已开发出一种混合免疫球蛋白玫瑰花环技术，用于检测抗猫肿瘤病毒相关细胞膜抗原的抗体。将感染猫白血病病毒的淋巴母细胞与待测血清孵育，然后用多聚甲醛固定。接着将其与用抗羊红细胞血清致敏的羊红细胞混合，以检测抗原-抗体反应；淋巴样细胞周围形成羊红细胞玫瑰花环表明其存在猫抗体。该方法的灵敏度比间接免疫荧光法至少高10倍。以感染猫白血病病毒的猫和狗细胞为靶标，混合免疫球蛋白玫瑰花环试验和间接免疫荧光试验显示出高度相关性。结果表明这两种试验可能检测的是相似反应。使用间接免疫荧光试验，我们发现75%的淋巴样肿瘤猫没有可检测到的抗体。用混合免疫球蛋白玫瑰花环技术检测了24份间接免疫荧光阴性的血清；其中至少7份血清显示有抗体。
