Calafat J, Weijer K, Hageman P C, Daams H
J Gen Virol. 1979 Oct;45(1):217-22. doi: 10.1099/0022-1317-45-1-217.
The indirect immunoferritin technique (IFT) that enables us to distinguish clearly whether an antibody reacts with a virus particle or only with the cell membrane, was used to study 25 cat sera and one rabbit anti-feline leukaemia virus (FeLV) serum using FL-74 cells as target. (1) All sera contained antibodies against FeLV even though 11 of the cats were viraemic at the same time; (2) from the effect of glutaraldehyde fixation of the FL-74 cells on the reaction with cat sera and the results of blocking experiments, it could be concluded that cat sera and rabbit anti-FeLV sera react partly with different antigenic specificities of FeLV, partly with the same antigens; and (3) the indirect membrane immunofluorescence test using FL-74 cells as target is not a good test to detect the presence of antibodies against feline oncornavirus-associated cell membrane antigen (FOCMA) because FL-74 cells produce a large quantity of FeLV and the fluorescence measured could be from antibodies against FeLV.
间接免疫铁蛋白技术（IFT）能够让我们清楚地辨别一种抗体是与病毒颗粒发生反应还是仅与细胞膜发生反应。该技术以FL - 74细胞为靶细胞，用于研究25份猫血清和一份兔抗猫白血病病毒（FeLV）血清。（1）所有血清都含有抗FeLV抗体，尽管其中11只猫同时处于病毒血症状态；（2）从戊二醛固定FL - 74细胞对与猫血清反应的影响以及封闭实验结果可以得出结论，猫血清和兔抗FeLV血清部分与FeLV的不同抗原特异性发生反应，部分与相同抗原发生反应；（3）以FL - 74细胞为靶细胞的间接膜免疫荧光试验不是检测抗猫肿瘤病毒相关细胞膜抗原（FOCMA）抗体存在的良好试验，因为FL - 74细胞会产生大量FeLV，测得的荧光可能来自抗FeLV抗体。
