Yin De-Ming, Wu Jin-Chuan, Yuan Ying-Jin
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P. O. Box 6888, Tianjin, 300072, PR China.
Biotechnol Lett. 2006 Jan;28(1):29-32. doi: 10.1007/s10529-005-4683-4.
Distinct spatio-temporal variations of metal ions and Taxol production were observed for Taxus cuspidata cells immobilized on polyurethane foam. The Taxol content in the inner foam layer reached 215 microg g(-1) at day 30, which was 40-fold higher than that in the outer foam layer, and the Ca2+ and Mg2+ contents were 5.3 and 3.7 times higher, while the K+ content was 5.5 times lower. Thus higher intracellular Ca2+ and Mg2+ contents and lower intracellular K+ content may favor the Taxol biosynthesis in immobilized Taxus cuspidata.
对于固定在聚氨酯泡沫上的东北红豆杉细胞，观察到金属离子和紫杉醇产量存在明显的时空变化。在第30天，泡沫内层的紫杉醇含量达到215微克/克，比外层泡沫层高40倍，Ca2+和Mg2+含量分别高5.3倍和3.7倍，而K+含量低5.5倍。因此，较高的细胞内Ca2+和Mg2+含量以及较低的细胞内K+含量可能有利于固定化东北红豆杉中紫杉醇的生物合成。
