Chang Zui, Guo Na, Liu Tong, Zhou Zhiqiang, Wang Yang
Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, China.
Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(3):294-8.
Paclitaxel and cephalomannine contents in wild Taxus cuspidata were determined by HPLC. The results indicated that paclitaxel and cephalomannine contents in T. cuspidate at the sunny side were slightly higher than that at the shadow side in the current-year and biennial branches. Paclitaxel and cephalomannine contents had no obvious regularity in leaves. Paclitaxel and cephalomannine contents were both the highest in the bark, then in the current-year branches, lower in the current-year leaves, and the lowest in the fruits. There were no remarkable correlation between stem diameter and paclitaxel and cephalomannine contents in the current-year branches and leaves. Significant difference was observed among samples collected in different period, and higher paclitaxel and cephalomannine concentrations were detected at the dormancy stage than that at the flower and fruit stages.
采用高效液相色谱法测定了野生东北红豆杉中紫杉醇和三尖杉宁碱的含量。结果表明，当年生和二年生枝条中，朝阳面东北红豆杉的紫杉醇和三尖杉宁碱含量略高于背阴面。叶片中紫杉醇和三尖杉宁碱含量无明显规律。树皮中紫杉醇和三尖杉宁碱含量均最高，其次是当年生枝条，当年生叶片中含量较低，果实中含量最低。当年生枝条和叶片的茎直径与紫杉醇和三尖杉宁碱含量之间无显著相关性。不同时期采集的样品间存在显著差异，休眠期检测到的紫杉醇和三尖杉宁碱浓度高于花果期。
