从细菌和酵母中极其快速地提取DNA。

Extremely rapid extraction of DNA from bacteria and yeasts.

作者信息

Cheng Hai-Rong, Jiang Ning

机构信息

Center for Microbial Biotechnology, Institute of Microbiology, Chinese Academy of Sciences, 100080, Beijing, China.

出版信息

Biotechnol Lett. 2006 Jan;28(1):55-9. doi: 10.1007/s10529-005-4688-z.

DOI:10.1007/s10529-005-4688-z

PMID:16369876

Abstract

A very simple and rapid method for extracting genomic DNA from Gram-negative bacteria, Gram-positive bacteria and yeasts is presented. In this method, bacteria or yeasts are lysed directly by phenol and the supernatant is extracted with chloroform to remove traces of phenol. The supernatant contains DNA that is suitable for molecular analyses, such as PCR, restriction enzyme digestion and genomic library construction. This method is reproducible and simple for the routine DNA extraction from bacteria and yeasts.

摘要

本文介绍了一种从革兰氏阴性菌、革兰氏阳性菌和酵母中提取基因组DNA的非常简单快速的方法。在该方法中，细菌或酵母直接用苯酚裂解，然后用氯仿提取上清液以去除痕量苯酚。上清液中含有适用于分子分析（如PCR、限制性酶切消化和基因组文库构建）的DNA。该方法可重复，且对于从细菌和酵母中常规提取DNA而言很简单。