Gohil Nisarg, Siruka Deepak, Musini Darshana, Makwana Kinjal, Khambhati Khushal, Bhattacharjee Gargi, Kalariya Ravi, Abusharha Ali, Ramakrishna Suresh, Singh Vijai
Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, 382715, Gujarat, India.
Department of Chemistry, School of Science, Indrashil University, Rajpur, Mehsana, 382715, Gujarat, India.
Appl Biochem Biotechnol. 2025 May 23. doi: 10.1007/s12010-025-05259-4.
Prodigiosin is a red-hued bacterial pigment with promising anticancer, antimalarial, antiparasitic, and antibacterial therapeutic properties. However, the commercial exploitation of this secondary metabolite is hindered due to high production cost and poor yield. In this study, the prodigiosin-producing Serratia marcescens SBL03 strain was isolated and identified. The pigment production capability was evaluated first in a complex commercial media, and thereafter in eight different agro-industrial waste materials as the sole source of nutrients. Among them, yellow mustard cake (YMC) medium titred 2.44 g/L prodigiosin, which was 7.62-fold higher than the higher pigment production achieved in the complex media, i.e., the nutrient broth (0.32 g/L of prodigiosin). The prodigiosin production was further enhanced through fermentation optimization exploring the Box-Behnken design (BBD) model and as high as 4.554 g/L titre of prodigiosin was achieved utilizing 1.06% w/v YMC at 28.3 °C temperature after 116 h and 42 min of incubation. This study is a small step towards a circular bioeconomy market with biovalorization of YMC medium for sustainable production of prodigiosin in a reasonable manner.
灵菌红素是一种具有红色色调的细菌色素，具有良好的抗癌、抗疟疾、抗寄生虫和抗菌治疗特性。然而，由于生产成本高和产量低，这种次生代谢产物的商业开发受到了阻碍。在本研究中，分离并鉴定了产灵菌红素的粘质沙雷氏菌SBL03菌株。首先在复杂的商业培养基中评估色素生产能力，然后在八种不同的农业工业废料作为唯一营养源的情况下进行评估。其中，黄芥末饼（YMC）培养基的灵菌红素产量为2.44 g/L，比在复杂培养基（即营养肉汤，灵菌红素产量为0.32 g/L）中获得的最高色素产量高7.62倍。通过探索Box-Behnken设计（BBD）模型进行发酵优化，进一步提高了灵菌红素的产量，在28.3°C温度下培养116小时42分钟后，利用1.06% w/v的YMC培养基，灵菌红素产量高达4.554 g/L。这项研究朝着循环生物经济市场迈出了一小步，即以合理的方式对YMC培养基进行生物增值，以可持续地生产灵菌红素。
