Matsson L
Department of Pedodontics, University of Umeå, Sweden.
Arch Oral Biol. 1992;37(6):445-50. doi: 10.1016/0003-9969(92)90098-s.
Two distinct types of mast cells are recognized in the rat: connective tissue mast cells (CTMCs), found in the peritoneal cavity, skin, tongue, etc. and mucosal mast cells (MMCs), found in the intestinal mucosa. The two subsets differ functionally and can be defined by histochemical methods. The aim here was to characterize the mast cell population in various oral mucosal sites. Biopsies were taken from the tongue, buccal mucosa, gingival mucosa and intestine (jejunum) of 20 rats. For optimal preservation of the MMCs, a fixative with low aldehyde concentration and low pH was used. The biopsies were embedded in paraffin. The first of three consecutive sections (5 microns) was stained with toluidine blue for 30 s, the second with toluidine blue for 7 days and the third with astra blue/safranine. Cells positive with toluidine blue after 30 s were classified as CTMCs, and those positive after 7 days but not after 30 s as MMCs. Cells positive to safranine in the astra blue/safranine staining sequence were classified as CTMCs and those positive to astra blue as MMCs. The total number of mast cells was similar in the superficial layers of all oral tissues studied. There were more mast cells in the deeper than in the superficial portions of the tongue. Mast cells with staining characteristics and size similar to those observed in the intestinal mucosa (MMCs) were found together with 'classical' connective tissue mast cells (CTMCs). The results suggest that the mast cell population of oral mucosal tissues of the rat contains both CTMC- and MMC-like cells.
结缔组织肥大细胞（CTMCs），存在于腹腔、皮肤、舌头等部位；以及黏膜肥大细胞（MMCs），存在于肠黏膜中。这两个亚群在功能上有所不同，并且可以通过组织化学方法进行定义。此处的目的是对不同口腔黏膜部位的肥大细胞群体进行特征描述。从20只大鼠的舌头、颊黏膜、牙龈黏膜和肠道（空肠）获取活检组织。为了最佳地保存MMCs，使用了低醛浓度和低pH值的固定剂。活检组织被包埋在石蜡中。连续的三个切片（5微米）中的第一个用甲苯胺蓝染色30秒，第二个用甲苯胺蓝染色7天，第三个用天青蓝/番红染色。30秒后对甲苯胺蓝呈阳性的细胞被归类为CTMCs，7天后呈阳性但30秒后不呈阳性的细胞被归类为MMCs。在天青蓝/番红染色序列中对番红呈阳性的细胞被归类为CTMCs，对天青蓝呈阳性的细胞被归类为MMCs。在所研究的所有口腔组织的表层中，肥大细胞的总数相似。舌头深层的肥大细胞比表层的更多。发现具有与在肠黏膜中观察到的染色特征和大小相似的肥大细胞（MMCs）以及“经典”的结缔组织肥大细胞（CTMCs）。结果表明，大鼠口腔黏膜组织中的肥大细胞群体同时包含CTMC样细胞和MMC样细胞。
